Gerald Cohen Publications (post-1985)

Refereed articles

21. G. O'Reagan, N. Sternberg and G. Cohen.
"Construction of an ordered overlapping library of bacteriophage P1 DNA in phage vector lambda D69".
Gene, 60, (1987), 129-135.
22. C. Keen, S. Mendelovitz, G. Cohen, Y. Aharonowitz and K. Roy. "Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus".
Mol. Gen. Genet., 212, (1988), 172-176.
23. D. Shiffman, M. Mevarech, G. Cohen, S. Jensen and Y. Aharonowitz.
"Cloning and comparative sequence analysis of the gene coding for isopenicillin N synthase in Streptomyces".
Molec. Gen. Genet., 214, (1988), 562-573.
24. G. Cohen and N. Sternberg.
"Genetic analysis of the lytic replicon of bacteriophage P1. I. Isolation and
partial characterization".
J. Mol. Biol., 206, (1989), 99-109.
25. N. Sternberg and G. Cohen.
"Genetic analysis of the lytic replicon of bacteriophage P1. II. Organization
of replicon elements".
J. Mol. Biol., 206, (1989), 111-133.
26. D. Shiffman, G. Cohen, Y. Aharonowitz, H. Palissa, H. von Dohren,
H. Kleinkauf and M. Mevarech.
"Isopenicillin N synthase gene of the unicellular gram-negative
Nucleic Acid. Res., 18, (1990), 660.
27. G. Cohen, D. Shiffman, M. Mevarech and Y. Aharonowitz.
"Microbial isopenicillin N synthases: structure, function, diversity and evolution".
Trends in Biotechnology, 8, (1990), 105-111.
28. G. Landan, G. Cohen, Y. Aharonowitz, Y. Shuali, D. Graur and D. Shiffman.
"Evolution of the microbial isopenicillin N synthase genes probably involved horizontal gene transfer".
Mol. Biol. Evol., 7, (1990), 399-406.
29. O. Landman, D. Shiffman, Y. Av-Gay, Y. Aharonowitz and G. Cohen.
"High level expression in Escherichia coli of isopenicillin N synthase gene from Flavobacterium and Streptomyces and recovery of active enzyme from inclusion bodies."
Fems Microbiol. Lett., 84, (1991), 239-244.
30. Y. Av-Gay, Y. Aharonowitz and G. Cohen.
"Streptomyces contain a 7.0 kDa cold shock like protein."
Nucleic Acid Res., 20, (1992).
31. Y. Aharonowitz, G. Cohen and J.F. Martin,
"Penicillin and cephalosporin biosynthetic genes: structure, organization, regulation and evolution."
Ann. Rev. Microbiol., 46, (1992), 461-495.
32. Y. Aharonowitz, Y. Av-Gay, R. Schreiber and G. Cohen.
"Characterization of a thioredoxin-like disulfide reductase from Streptomyces clavuligerus and its possible role in ß-lactam biosynthesis."
J. Bacteriology, 175, (1993), 623-629.
33. G. Newton, R. Fahey, G. Cohen and Y. Aharonowitz.
"Low molecular weight thiols in streptomycetes and their potential role as antioxidants."
J. Bacteriology, 175, (1993), 2734-2742.
34. G. Cohen, M. Yanko, M. Mislovati, A. Argaman, R. Schreiber, Y. Av-Gay and Y. Aharonowitz.
"Thioredoxin-thioredoxin reductase system of Streptomyces clavuligerus: sequences, expression and organization of genes."
J. Bacteriology, 175, (1993), 5159-5167.
35. Y. Aharonowitz, J. Bergmeyer, J. Cantoral, G. Cohen, A. Demain, U. Fink, H. Kinghorn, H. Kleinkauf, A. MacCabe, H. Palissa, E. Pfeifer, T. Schwecke, H. van Liempt, H. von Dohren, S. Wolfe and J. Zhang.
"ACV synthetase, the multienzyme integrating the four primary reactions in beta-lactam biosynthesis, as model peptide synthetase."
Bio/Technology, 11, (1993), 807-810.
36. G. Cohen, A. Argaman, R. Schreiber, M. Mislovati and Y. Aharonowitz.
"Thioredoxin system of Penicillium chrysogenum and its possible role in penicillin biosynthesis."
J. Bacteriology, 176, (1994), 973-984.
37. I. Sanyal, G. Cohen and D. Flint.
"Biotin synthase: Purification, characterization as a [2Fe-2S] cluster protein, and in vitro activity of the Escherichia coli bioB gene product."
Biochemistry, 33, (1994), 3625-3631.
38. G. Newton, C. Bewley, T. Dwyer, R. Horn, Y. Aharonowitz, G. Cohen,
J. Davies, D. Faulkner and R. Fahey.
"The structure of U17 isolated from Streptomyces clavuligerus and its properties as an antioxidant thiol."
Eur. J. Biochemistry, 230, (1995), 821-825.
39. K. Gibson, G. Lorimer, A. Rendina, W. Taylor, B. Wexler, G. Cohen, A. Gatenby, W. Payne, D. Roe, B. Lockett, E. Marsilii, I. Turner, C. Kalba and H. Chi.
"Dethiobiotin synthetase: the carbonylation of 7,8-diaminononanoic acid proceeds regiospecifically via the N7-carbamate".
Biochemistry, 34, (1995), 10976-10984.
40. I. Borovok, O. Landman, R. Kreisberg, Y. Aharonowitz and G. Cohen.
"The ferrous active site of isopenicillin N synthase: genetic and sequence analysis of the endogenous ligands."
Biochemistry 35, (1996), 1881-1887.
41. Newton, K. Arnold, M. Price, C. Sherill, S. Delcardayre, Y. Aharonowitz, G. Cohen, J. Davies, R. Fahey and C. Davis.
"Distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes".
J. Bacteriology. 178, (1996), 1990-1995.
42. G. Cohen, E. Or, W. Minas and N. Sternberg.
"Directionality of bacteriophage P1 replication and role of cis-acting elements".
Gene. 175 (1996), 151-155.
43. O. Landman, I. Borovok, Y. Aharonowitz and G. Cohen.
"The glutamine ligand in the ferrous active-site of isopenicillin N synthase of Streptomyces jumonjinensis is not essential for catalysis".
FEBS Letters 405 (1997), 172-174.
44. S. Liu, S. Sela, G. Cohen, J. Jadoun, A. Cheung and I. Ofek.
"Genetic evidence for co-regulation of Streptolysin S production and riboflavin biosynthesis in Streptococcus pyogenes".
Microbial Pathogenesis. 22 (1997) 227-234.
45. O. Gal-Mor, I. Borovok, Y. Av-Guy, G. Cohen and Y. Aharonowitz.
"Gene organization in the trxA/B - oriC region of the Streptomyces coelicolor chromosome and comparison with other eubacteria".
Gene. 217 (1998), 83-90.
46. R. Kreisberg-Zakarin, I. Borovok, M. Yanko, F.Frolow, Y. Aharonowitz and G. Cohen.
"Structure function studies of the non - heme iron active site of isopenicillin N synthase: some implications for catalysis".
Biophysical Chemistry (2000), 86, 109-118.
47. G. Cohen, I. Borovok, O. Uziel, R. Schreiber and Y. Aharonowitz.
"Bacterial thiol-disulfide redox metabolism: A new target for rational drug design".
Periodicum Biologorum (2001), 103, 153-155.
48. D. Govorko, G. Cohen and B. Solomon
"Single-chain antibody against the common epitope of mutant p53: isolation and intracytosolic expression in mammalian cells."
Journal of Immunological Methods (2001), 258, 169-181.
49. I. Borovok, R. Kreisberg-Zakarin, M. Yanko, R. Schreiber, M. Myslovati, F. Aslund, A. Holmgren, G. Cohen and Y. Aharonowitz.
"Streptomyces contain class Ia and class II ribonucleotide reductases: expression analysis of the genes in vegetative growth."
Microbiology (in press).
50. M. Masalha, I. Borovok, R. Schreiber, Y. Aharonowitz and G. Cohen.
"Transcriptional Analysis of the Staphylococcus aureus Aerobic Class Ib and Anaerobic Class III Ribonucleotide Reductase Genes in Response to Oxygen."
J. Bacteriology (2001), 183, 7260-7272
51. M. Paget, V. Molle, G. Cohen,Y. Aharonowitz and M. Buttner.
"Defining the disulfide stress response in Streptomyces coelicolor A3(2): identification of the oR regulon."
Molecular Microbiology (2001), 42, 1007-1002
Chapters in Books
52. Y. Aharonowitz and G. Cohen.
"Bioactive secondary metabolites." In: Biotechnology: Potentials and Limitations. Ed. S. Silver and P. Abelson. Report of Dahlem Workshop, pp. 99-112, Berlin, Springer Verlag, Berlin, (1985).
53. G. Cohen and Y. Aharonowitz.
"Molecular genetics of antimicrobials: A case study of beta-lactam antibiotics."
In: The Society of General Microbiology Symposium, Fifty Years of Antimicrobials, pp. 139-163, Cambridge University Press, (1995).
54. A. Gargir, S. Liu, S. Sela, G. Cohen, J. Jadown, A. Cheung and I. Ofek.
"Biological significance of the genetic linkage between streptolysin S expression and riboflavin biosynthesis in Streptococccus pyogenes".
Adv. Exp. Med. Biol. 418, (1997) 987-989.
55. G. Cohen.
"Introduction to the Genetics of Industrial Microorganisms".
In: Manual of Industrial Microbiology and Biotechnology, 2nd edition (Eds. A.Demain and J. Davies), American Society for Microbiology, ASM Press, Washington DC.
56. R. Kreisberg-Zakarin, I. Borovok, M. Yanko, Y. Aharonowitz and G. Cohen.
"Recent advances in the structure and function of isopenicillin N synthase"
In: Antonie van Leeuwenhoek: Biosynthesis of beta -lactam antibiotics. 75 (1999), pp 33-39, Kluwer Academic Publishers, The Netherlands.
1. Y.Aharonowitz, L. Van der Voort, G. Cohen, R. Bovenberg, R. Schreiber, A. Argaman, Y. Av-Gay, H. Nan, A. Kattevilder, H. Palissa and H. Van Liempt.
"An oxido reductase enzyme system obtainable from P. chrysogenum, the set of genes encoding the same and the use of oxido reductase enzyme systems or genes encoding the same for increasing antibiotic production".
European Patent Application No. 91201542.7
Publication No. EP 0-462-674-A1.
Date of filing 18.06.91.
2. Y.Aharonowitz, L. van der Woort, G. Cohen, R. Bovenberg, R. Schreiber, A. Argaman, Y. Av-Gay, H. Nan, A. Kattevilder, H. Palissa and H. van Liempt.
"Oxido reductase enzyme system obtained from P. chrysogenum.
U.S. Patent No. 5,328,839
Date of Patent: 12.07.94.
3. O.Uziel, I. Borovok, G. Cohen and Y. Aharonowitz.
"Recombinant Staphylococcus aureus thioredoxin reductase, the use of the enzyme system or genes encoding the same as targets for new antimicrobial drugs development and its inhibitors as antimicrobial agents".
U.S. Patent Application pending.
4. D.Govorko, G. Cohen and B. Solomon.
"Construction of a single chain antibody (scFv) against the conserved epitope of mutant p53 protein in human tumors and its application for cancer therapy".
U.S. Patent Application pending.


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