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| Growth on BCYE media with gray-white colonies. Though there are more than 40 species of Legionella, L. pneumophila is the major pathogen. L. pneumophila is a fastidious, nonsporeforming, aerobic, gram-negative, slender rod. It can be grown on selective BCYE media and confirmed with immunofluorescent staining.
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| Direct fluorescent antibody (DFA) testing with fluoresceinated antibody specific for Legionella can be directly performed on sputum samples |