| Gram Stain | |
 Large spore forming rods | |
| Blood agar growth (anaerobic) | |
 | |
| small colonies with double zone of hemolysis | |
 | |
| Fatty acid peaks for acetic acid, proprionic acid, and butyric acid, but little or no isobutyric or isovaleric acid | |
| Clostridium perfringens | |
| Medically important Clostridium species | |
| C. perfringens - cause for wound infection with gas gangrene | |
| C. tetani - cause for tetanus | |
| C. botulinum - cause for botulism from food poisoning | |
| C. difficile - cause for antibiotic-associated enterocolitis | |