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National Laboratory for the Genetics
of Israeli Populations

NLGIP Symbol

EBV Immortalization Service

Our lab can prepare EBV-immortalized B-cell lines for you.

  • Our lab prepares EBV-immortalized B-cell lines from fresh blood samples.
  • EBV-immortalized B-cell lines are an indefinite source for genomic DNA, mRNA and proteins from a selected individual.

  • The price for this service is US$240 per blood sample (excluding shipment; no payment in case of failure to immortalize the cells).
  • A sample of at least 5 ml fresh uncoagulated blood is required for same-day shipping.
  • A sample of at least 10 ml is needed when the blood is shipped on the following day after sampling.
  • We recommend using Heparin (green cap) Vaccutainer tubes.
  • Please mix the fresh blood gently to ensure the absence of microscopic thrombi.
     
  • The blood must reach us within 48 hours after sampling.
  • The blood sample must be sent at ambient temperature of 20 – 30 degrees Celsius. Avoid cooling with ice, but avoid heating beyond 30 degrees (use of aluminum foil and thermal isolation is recommended)

  • When the last two criteria cannot be met, we suggest isolating lymphocytes from the blood samples; freezing in 70% RPMI/20% FCS/10% DMSO; shipping with dry ice. (whole blood cannot be frozen without loosing lymphocyte viability)
  • Typical periods for establishing a B-cell line are 8-12 weeks.
  • Larger blood volumes and faster shipments increase the chances for establishing cell lines from your samples.

Shipping Address:
National Laboratory for the Genetics of Israeli Populations
Sackler Faculty of Medicine; Room 1017
Tel-Aviv University
Tel-Aviv 69978, Israel

 

For drivers: entry is from Gate 1 (north-east corner) of the Tel-Aviv University campus, then take the first right turn to the Sackler building

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Contact Person: David Gurwitz <gurwitz@post.tau.ac.il>; Voice/FAX +972-3-640-7611