LS6500 Liquid Scintillation Counter
LS 6500 Scintillation Counter combine advanced scintillation technology, configurability to meet your exact requirements and built-in upgradability to add features, as you need them. Value Systems are instrument configurations that meet standard requirements, H-Number Plus, our patented quench quench monitor, and Versa-RackT are standard features on both LS 6500 Value Systems. You can count samples in any combination; up to 336 standard vials (20 mL).
The isotope library C 14, H3, I125, .S35 and P32 are permanently stored in the system.
The biolistic PDS-1000/He unit. Top, main chamber
containing the micro carrier launch assembly and the bombardment helium
pressure gauge. The central gauge (in the left side of the instrument) monitors
the vacuum within the chamber, and the 2 lower knobs adjust the vacuum flow and
vent rates. The Helium metering valve is next to the main chamber.
PDS-1000 | He System
Transform Any Cell Type
The biolistic PDS-1000/He instrument is capable of accelerating sub cellular-sized tungsten or gold microprojectiles coated with DNA over a range of velocities and target distances necessary to optimally transform many different cell types. Particle delivery with the PDS-1000/He system is a convenient method for transforming intact cells in culture since very little pre- or post bombardment manipulation is necessary, and sample containment within the evacuated bombardment chamber offers maximum control.Biolistic bombardment allows transformation of animal cells with unique growth requirements that are not amenable to gene transfer using any other method. Reports describing how to transform cultured mammalian cells with the PDS-1000/He device (see References) show improved transient transformation of a variety of sensitive mammalian cells that are poorly transformed by chemicals, lipofection, or even electroporation. In addition, you can use less DNA and fewer cells for either transient or stable transformation with the biolistic particle delivery system. This technique is much easier and faster than the tedious task of microinjecting embryos. How the Unit WorksThe helium pressure and vacuum circuits in the PDS-1000/He system effectively accelerate the microcarriers into the target cells: After all the materials are in place, the chamber door is closed and a vacuum is applied. The vacuum reduces the frictional drag on the DNA-coated microcarriers and provides a safety interlock; the instrument cannot be activated unless a vacuum is drawn.Activating the fire switch allows helium to flow into the gas acceleration tube at a rate regulated by the helium-metering valve and monitored by the helium pressure gauge. The gas is held until the burst pressure of the rupture disk is reached. This generates a helium shock wave into the bombardment chamber.The shock wave hits the microcarrier launch assembly and propels a plastic macrocarrier holding DNA-coated microcarriers toward the target cells. A stopping screen placed between the macrocarrier assembly and the cells retains the plastic disk, while allowing the coated microprojectiles to pass through and transform the target cells.Bombardment ParametersWe recommend these settings for bombardment of a variety of cell types. Many factors affect bombardment efficiency, but most users will find it sufficient to optimize the major variables individually, then test their interactions on a limited scale.
||Helium Pressure (psi)
||0.6 Ám gold
||0.6 Ám gold
||1.0 Ám gold
|Callus or cell cultures
||1.0 Ám gold
|Sub cellular organelles
||0.6 Ám gold
||1.6 Ám gold
||1.6 Ám gold
Argon Laser: 458nm , 488nm and 514nm
HeNe: 543nm and 633nm
The Aviv Model 215 Circular Dichroism Spectrometer
Circular dichroism spectroscopy measures physical phenomena wherein molecules absorb to different extents circularly polarized light. Differences in the absorbance are directly related to the optical activity of chiral molecules.
Circular dichroism is an essential tool of measuring secondary structure of proteins, ligand binding and macromolecular association. Deconvolution of the spectra can give a quantitive measure of protein secondary structure.
The Aviv model 215 Circular Dichroism Spectrometer records circular dichroism as a function of wavelength, time, temperature, pH and concentration using a double monochromator containing two UV grade prisms as dispersing elements. Focusing optics allows use of micro cells. A 50 Khz photoelastic modulator produces right and left circularly polarized light.
The instrument allows secondary structure estimates, stopped-flow kinetic studies, wavelength scans, temperature scans, titration studies, total fluorescence measurements, and more. Wavelength ranges 165nm to 900nm .
MultiphorT II flatbed
Two-dimensional (2-D) electrophoresis is a powerful analytical technique capable of resolving thousands of individual proteins in complex biological samples. In cases where the resolution provided by larger-format systems is not required, the speed and convenience of a mini-format can be exploited.
The Multiphor T II Flatbed Electrophoresis Unit allows both the first and second dimensions of 2-D electrophoresis to be performed with the same instrument. Three second-dimension separations are run simultaneously on a single second-dimension gel, which is particularly useful for comparative studies, as all three samples experience identical separation conditions. Silver staining using the PlusOne T Silver Staining Kit, Protein; and the Hoefer T Automated Gel Stainer provides exceptionally sensitive visualization when applied to the
ExcelGel T precast gel used in this technique.
Multiphor II electrophoresis system is a versatile modular system of Pharmacia for horizontal electrophoresis, isoelectric focusing 2-D electrophoresis and electrophoretic transfer.
Zeiss upright fluorescence microscope
The filters are:
1)UV filter, EX 365, EM 420.
2)Green filter, EX 490 EM 525.
3)Red Filter,EX 540 EM 580.
Leica manufactured DMRB upright microscope with magnafire 12-bit color CCD camera.
The filters are:
1)B.G.R filter for uv, blue and green, excitation filter, BP 400/20, Dichromatic mirror rkp 415, Suppression filter BP 465/20.
2)DAPI excitation filter BP 340-380, Dichromatic mirror rkp 400, Suppression filter LP 425.
3) M2(Rhodamin) excitation filter BP 546/14, Dichromatic mirror rkp 580, Suppression filter LP 590.
4) Gfp excitation 470/40x, Dichromatic mirror hq495LP, em hq 525/50m.
Packard RIASTAR multi well gamma counter
5 well multi well gamma counter with multi channel analyzer technology The instrument is configured with 10 detectors.
Packard RiaStar Gamma Counting Systems includes multi -channel analyzer, Instrument Performance Assessment (a self-inspecting system) and automatic protocol operating.
HPLC- High Performance Liquid Chromatography
The water's hplc includes 50u pumps model 626, fluorescence detector 474, PDA detector 996, 600s controller, vacuum degasses and non-cooled auto sampler 717.
The instrument is fully controlled by the millennium software.
The Turner Designs model 20e is equipped with a Hamilton injector for automatic injections.
Its EPROM includes auto ranging and 2nd functions for smoothing, pre delay, delay, repeat, and continuos reading.
The most sensitive display range is 0.000 to 9.999 and the least sensitive display range is 0000 to 9999.
Microplate Reader Spectramax 190
The SPECTRAmax 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. For aqueous solutions, our patented* PathCheck« sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The corrected absorbance is within 5% of the value obtained in a conventional spectrophotometer
The SPECTRAmax 190 microplate reader allows you to:
Use the extinction coefficient of your sample to calculate concentrations directly,
Correct for volume differences in the wells, expand the dynamic range of your assay,
Detect pipetting errors and check performance of your pipetting devices
Any molecule that can be resolved in a slab gel can be purified quickly, easily, and completely using continuous elution electrophoresis. Using SDS-PAGE, native PAGE, or agarose gel electrophoresis, the Model 491 prep cell and mini prep cell isolate individual components from crude or partially purified samples. These instruments are ideal for use at any stage in a purification scheme. As a final step following chromatography or preparative isoelectric focusing, these instruments can be used to isolate a specific molecule from any contaminants remaining in the sample.
Ě Conventional electrophoresis buffer systems and media are used
Ě Purify nanograms to milligrams of a particular molecule
Ě Using SDS-PAGE, purify molecules that differ in molecular weight by as little as 2%
Ě Using native PAGE, isolate molecules that differ in isoelectric point by as little as 0.1 pH unit.
Purified molecules are automatically collected in individual liquid fractions and are immediately available for sequence analysis, crystallography, antibody production, enzyme kinetics studies, NMR, bioassays, or any other study where homogeneous bio-molecules are required. Single- and double-stranded DNA and RNA can also be purified using the prep cell.
Protein Purification Simplified
The Rotofor system fractionates complex protein samples in free solution using preparative isoelectric focusing. This nondenaturing technique rapidly isolates proteins of interest from the bulk proteins in complex biological samples. With the addition of the mini Rotofor focusing chamber, this unique system accommodates a wide range of sample volumes and sizes.
Sequencer ABI 377 and 3100
Ask Dr. Eli Geffen
Spectrophotometer Ultrospec 2000
Pharmacies ultrospec 2000 UV/visible spectrophotometer measures
standard absorbance, concentration and transmittance and has stored parameters
for DNA, RNA and oligonucleotide quantification and purity checking as well as
for protein contamination measurements in nucleic acid solutions.
Kinetic is also possible with 6 cell changer.
Bandwidth of 3nm and stray light at 220nm < 0.012%T.
Ultracentrifuge - OptimaT LE
The Beckman optima le70 is equipped with the following rotors: SW 28, SW41, SW50.1, SW60 and 70, 70.1Ti.
The max rpm are 70,000 and max vacuum is less than 20 microns
Elutriation Centrifuge -Avanti« J-25
The Avanti series is a centrifuge that allow the centrifugal elutriation of living cells
Cells are subjected to two forces within the separation chamber: the centrifugal field and the viscous drag of the fluid following in the opposite (centripetal) direction. Each cell tends to migrate to a zone where its sedimentation rates are balanced by the flow rate of the fluid through the separation chamber.
By increasing the flow rate of the elutriation fluid in steps or decreasing the rotor speed, successive populations of relatively homogenous cell sizes can be washed from the chamber.
ImageMaster 1D Image Analysis Software
Image analysis is an integral part of most of today's Life Science applications and at APB we have the most comprehensive range of software products available. Under the Image Master label, we cater for all your analysis needs, from the basic documentation of a routine purity screen to the querying of entire projects of 2D gels for discovering protein expression changes.
This site covers all your analysis needs for applications which include band and spot quantitation, the study of complex band pattern matching in 1D gels, examination of lane relationships between many gels, high throughput screening techniques aimed at 2D proteomics projects and rapid comparison of macro arrays.
Combined with the large range of image capture devices we offer for all types of detection methods, we can assemble a complete system for exactly the application with which you work, whether it be DNA or proteins, arrays, 1D or 2D gels, labeled or stained.
Umax scanner and two software for densitometry developed by Pharmacia enabling quantitation of 1D gels and 2D gels
"PAM-Fluorometer Family" employs the so-called Pulse-Amplitude-Modulation (PAM) measuring principle, which is unique in providing a highly selective measure of the relative chlorophyll fluorescence quantum yield. In conjunction with the so-called "saturation pulse method" of fluorescence quenching analysis PAM-2000 -Fluorometers allow rapid assessment of photosynthetic energy conversion. Measuring principle and quenching analysis
The Portable Chlorophyll Fluorometer PAM-2000 has been developed on the basis of the well-known PAM Fluorometer. The patented pulse amplitude modulation (PAM) measuring principle is used to measure the fluorescence yield with high
Sensitivity and selectivity, whereby even strong unmodulated light (e. g. full sunlight and saturation pulses with 10000 Ámol quanta ms) causes no disturbing effects.
The intensity of the measuring light is sufficiently low that it has no effect on the Photosynthesis State and the fluorescence yield. In addition to the current fluorescence yield (Ft, in continuous light) and the maximum yield (Fm', in the saturation pulse) it is also possible to determine the minimum yield (Fo', in the quasi-dark state). This information is automatically evaluated by the data acquisition system integrated in the PAM-2000 Fluorometer such that the quenching coefficients (qP and qN), the effective quantum yield (F/Fm') and the apparent electron transport rate (PAR x F/Fm') are obtained within seconds following each saturation pulse
IPGphor- Isoelectric Focusing System
IPGphor Isoelectric Focusing System is a dedicated instrument for performing immobilized pH gradient (IPG) IEF. IPGphor delivers rapid, high-resolution first dimension protein separations with pre-cast Immobiline Dry Strip IPG gels.
The integrated system includes a programmable 8,000 V, 1.5 mA power supply and Peltier solid-state temperature control (18-25░C ▒ 1 ░C) for up to 12 IPG strips.
IPGphor features an innovative strip holder, made out of aluminum oxide and platinum, which allows reswelling of IPG strips and subsequent IEF separation without handling the strips between steps. For examples of results obtained using IPGphor Isoelectric Focusing System.
The ABI Prism« 7000 Sequence Detection System is a complete, real-time PCR system that detects and quantitates nulceic acid sequences. In real-time PCR, cycle-by-cycle detection of accumulated PCR product is made possible by combining thermal cycling, fluorescence detection, and application-specific software in a single instrument. Quantitative results are available immediately after PCR without additional purification or analysis.
Real-time, quantitative PCR applications include gene expression and pathogen detection.Post-PCR detection is also available for non-quantitative assays such as allelic discrimination (SNP detection) and plus/minus assays.
Affymetrix 428 Array Scanner
The Affymetrix 428 Array slides Scanner is a digital confocal laser scanning epifluorescent microscope for viewing fluorescent-dye tagged samples.
The scanner is equipped with two lasers : 532nm and a 635nm (cy3 and cy5).
Data Mining Tool (DMT).
The Data Mining Tool (DMT) software provides a variety of tools for filtering and sorting GeneChip« array data enabling you to find your most significant data results quickly and easily. Key features include:
DMT is compatible with Windows NT and Windows 2000 operating systems.FTIR
Fourier transform-infrared (FT-IR)
Fourier transform infrared (FT-IR) is a technique that uses an interferometer for data collection and a digital Fourier transformation to process the data.
TheNexus FT-IR spectrometer family provides superior performance to meet the demanding infrared sampling requirements. Design excellence and smart system components ensure the Nexus will provide reproducible and accurate results. The Nexus provides real-time feedback on all aspects of sample analysis with exclusive Enhanced Synchronization Protocol (E.S.P.) technology.
The Nexus is Thermo Nicolet's research-grade, fully upgradeable FT-IR spectrometer. The Nexus is the highest-performance spectrometer with superior design, ease of use and total flexibility.
The Nexus takes full advantage of Thermo Nicolet's unique Smart System technology that features automatic purge, optical component recognition and much more, providing unsurpassed accessibility to research level results! Every Nexus system links all aspects of the system together so that experiments are optimized. A Nexus spectrometer automatically configures sampling parameters, beam path, spectral range and experiment types. Snap a Smart Accessory into your Nexus and you will find uncompromising sampling performance. Thermo Nicolet Smart Accessories provide the highest optical throughput, reliability and sampling flexibility. OMNIC software provides the interface that makes the Nexus smart system work. Our wide range of add-on experiments, coupled with expanded spectral range, give spectroscopists options available with no other instrument.
All Nexus spectrometers can be configured for multiple spectral ranges. Changing spectral range is as easy as removing an optical component and placing the new one into its precision, pinned position. Nexus automatically updates ranges, sensitivity and parameters. Thermo Nicolet is the only FT-IR manufacturer with the proven ability to upgrade from a basic system to a fully advanced research system. The universal optics and electronics platforms make Nexus spectrometers flexible in every sense of the word. Nexus components, including optics, are pinned-in-place, which ensures they are always in alignment as well as guaranteeing reproducibility, stability and the ability to swap and replace components.
The Nexus spectrometer has the most efficient optical path of any infrared spectrometer, providing excellent quality spectral data. Nexus systems utilize continuous dynamic alignment to ensure exceptional high-resolution lineshapes, and provide superior
FT-IR spectrometers record the interaction of IR radiation with experimental samples, measuring the frequencies at which the sample absorbs the radiation and the intensities of the absorptions. This is a single beam instrument.
Detector-DTGS (KBr Window): deuterated triglycine sulfate. A very sensitive detector for mid infrared range measurement. Most routine scanning instruments feature the more expensive and more sensitive DTGS detector, which is one or two orders of magnitude more sensitive than lithium tantalate, depending upon implementation. One drawback of the DTGS detector in a process environment is that it stops functioning as a detector at temperatures higher than 41 degrees C; the detector depolarizes above this temperature.
Mid ***** 3.00 - 6.00 microns (3000-6000 nanometer), Beamsplitter***KBr Spectral Range(cm-1)= 7400-350
PIT PIT tag (Passive Integrated Transponder) externally mounted or implanted RFID (Radio Frequency Identification) product used to positively identify animals and other objects (rocks, golf balls, driftwood, etc.) for a lifetime. Each tag is a read-only tag that is programmed to transmit a unique code only when activated. This code cannot be changed. There are tags that can be programmed but Biomark, Inc. does not offer that product nor do we recommend it for animal identification .
Academic and pharmaceutical researchers commonly use the mouse for
investigations of genetics, cellular physiology, and of agents affecting bone
or soft-tissue composition. Bone densitometry and body composition measurement
using dual-energy x-ray absorptiometry (DEXA), eliminate the need for
destructive chemical analysis - a time- and labor-consuming process that
requires days or weeks to complete. Bone densitometry allows the researcher the
opportunity to make multiple measurements in-situ during the life of the
animal. Unfortunately, DEXA densitometers using rectilinear scanning require as
much as 30 minutes for a total body acquisition with relatively poor precision
(>5%) and spatial resolution (>0.5mm). The long process requires careful
sedation techniques and often endangers animal safety. The PIXImus densitometer
provides bone mineral and body composition results from total body imaging in
under 5 minutes. Fast imaging allows use of milder sedation that is safer on
animals.The PIXImus allows accurate and precise measurement of bone and tissue
for small animals 10-50 g (e.g.,mice, lemmings). This measured bone exhibits
excellent correlation to total ashed weight (r=0.99). PIXImus uses a lower
x-ray energy than that used for peripheral densitometry in humans in order to
achieve contrast in the extremely low density bone. Excellent precision of BMD
and %Fat makes PIXImus ideal for longitudinal studies. The standard total body
focuses on the active bone area in the subcranial region. There are manual
ROI's for selected regions within the total body image, such as spine and
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Features Detection limit of 3 x 10-21 moles of luciferase using
Promega's Luciferase Assay Reagent
When making fluorescence determinations, the Synergy HT uses a tungsten halogen
lamp with interference filters: VP-ITC
X-ray Diffraction The possibility of using
powder diffraction methods to study materials was recognized shortly after the
discovery of X-ray diffraction by von LaŘe and Knipping in 1910. In particular,
the construction of a simple powder diffractometer was described by Hull in
1917, and the instrument was used to obtain patterns from a number of simple
materials such as diamond, graphite and iron. Within a few years, many others,
including the Braggs and Pauling, had exploited the powder method to study a
wide range of materials, including metals, minerals, and simple organic solids
X-ray Diffraction System for
Protein Crystals X-Ray1 1. Rigaku (manufacturer); ultraX18 (18kW rotating anode x-ray
generator); Raxis-IV Image Plate area detector;
confocal "blue" OSMIC X-ray mirrors. X-Ray2-NEW 2 . Rigaku (manufacturer); MicroMaxTM-007 HF
(800W rotating anode microfocus x-ray generator);
Image Plate area detector; VariMaxTM focusing X-ray mirrors. LSM_META
Detection limit of 3 x 10-21 moles of luciferase using Promega's Luciferase Assay Reagent
When making fluorescence determinations, the Synergy HT uses a tungsten halogen lamp with interference filters:
The possibility of using powder diffraction methods to study materials was recognized shortly after the discovery of X-ray diffraction by von LaŘe and Knipping in 1910. In particular, the construction of a simple powder diffractometer was described by Hull in 1917, and the instrument was used to obtain patterns from a number of simple materials such as diamond, graphite and iron. Within a few years, many others, including the Braggs and Pauling, had exploited the powder method to study a wide range of materials, including metals, minerals, and simple organic solids .
X-ray Diffraction System for Protein Crystals
1. Rigaku (manufacturer); ultraX18 (18kW rotating anode x-ray generator); Raxis-IV Image Plate area detector;
confocal "blue" OSMIC X-ray mirrors.
2 . Rigaku (manufacturer); MicroMaxTM-007 HF (800W rotating anode microfocus x-ray generator);
R-AXIS IV++ Image Plate area detector; VariMaxTM focusing X-ray mirrors.
The Beam Path:
The NanoDrop ND-1000 UV-Vis Spectrophotometer enables highly accurate analyses of 1 ul samples with remarkable reproducibility. The patented sample retention system eliminates the need for cuvettes and capillaries which decreases the measurement cycle time. In addition, the high absorbance capability eliminates the need for most dilutions. You'll find yourself making fewer dilutions and taking more readings than ever before, essentially becoming a more quantitative laboratory.
The ND-1000 offers many benefits: Small samples: designed for 1 ul samples Large dynamic range: measures 2-3700 ng/ul< (dsDNA) on a single sample. No need to change out cuvettes or caps. High absorbance capability: 50 times that of traditional spectrophotometers No Dilutions for most samples No extra costs for consumables Full Spectrum (220-750nm) 10 second measurement time No cuvettes or capillaries Sample is recoverable Small size (size of a tissue box) Easy set up (plug and play) Software designed for life sciences Free software upgrades
The system ensures radioisotopic imaging with high sensitivity, which comes from Fujifilm's unique and reliable technologies. Imaging plate (IP) consists of a radiosensitive layer of photostimulable phosphor crystals on a polyester support. The IP accumulates irradiated radiation energies on exposure for further scanning excitation process. The innovation and ergonomic design provides a broad range of advantages.
FLA-5100 has three kinds of excitation laser beams: 473nm, 532nm and 635nm. The system has four filters (510nm, 575nm, 665nm, and a filter for IP) for upgrading flexibility.
18-angle light scattering detector for the measurement of absolute molecular weight, size, and conformation of macromolecules in solution. The DAWN« HELEOST II may be used in batch mode (off-line) or connected on-line to an HPLC/HPSEC/AFFF, etc.. The DAWN« HELEOST II utilizes a 120mW solid-state laser operating at 658 nm and also has on-board digital signal processing hardware for up to four external devices such as RI and/or UV detectors. Also contains an analog output from the 90░ detector for interfacing to strip chart recorders. The DAWN« HELEOST II has a gorgeous 64,000 color LCD display, thermostatic control options, depolarization options, and the ability to interface to high temperature GPC systems like the PL210 and Waters Alliance 2000. On-board analog-to-digital conversion with Ethernet communication for data acquisitionOptilab T-rEX
The Optilab« rEX (refractometer with EXtended range) is an Refractive Index
detector with 256 times the detection power and up to 50 times the
dynamic range of every RI detector in existence today. Using a
combination of cutting-edge semiconductor photodiode technology and proprietary
computer algorithms, the rEX presents a host of instrument firsts.
For example, there are no range or gain settings. The full range of instrument detection is always present, and the full sensitivity exists over the entire range.
The rEX also has a flow cell with a total volume of only 7.4 uL -- 25% less than the leading refractometers available today. This translates into minimized band broadening and better temperature stability.
The Optilab« rEX can also do something no other on-line Refractive Index detector can: it can measure the absolute refractive index of a solution. In addition, it can measure the dn/dc of a solvent at the same wavelength of light as the light scattering instrument. And just to make sure it can't be matched, you can replace the light source in the rEX for other wavelengths (e.g. 690, 633, 488, etc.)!
The new Optilab« rEX shares the ability its predecessor (the Optilab« DSP) to go to below ambient temperatures as easily as above ambient. No hassle temperature control can be programmed down to 4░C or as high as 50░C. Compatible with all HPLC systems.
Two Lasers: 488 and 630
Software: Flow Jow
Xe arc, air-cooled
150W Hg-Xe arc, air-cooled (optional)
|Monochromator||F/7 double prism, both dispersive, both polarising|
▒0.2 nm (170nm - 400nm), ▒0.5 nm (400nm - 900nm)
▒0.05 nm (170 - 400 nm), ▒0.1 nm (400 - 800 nm)
|Wavelength resolution||0.1nm throughout range.|
0 to 2nm at 160nm, 0 to 4nm at 180nm, 0 to >10nm at >250nm
165nm - 1360nm.
<5ppm at 200nm, <8ppm at 180nm
|Baseline stability||▒0.02 m o /hr|
Discrete sampling step-scan, Adaptive sampling step-scan
|Scanning speeds||Dependent upon step-scan resolution approximately 500nm min-1 at 0.5nm step|
Linear timebase, Split-linear timebases, Logarithmic timebase
|Response time||Not applicable. Chirascan uses the superior method of digital oversampling which eliminates the risk of damage to data.|
|CD full scale||▒1500 m|
|CD resolution||64 bit|
0.015m ░ at 185nm, 0.012m ░ at 200nm, 0.015m ░
at 500nm, (1nm bandwidth, 16s oversampling (integration))
Internal: 2 standard; up to 16 optional.External (standard): 6 inputs for temperature (multiplexed); 3 analogue inputs; plenty of digital I/O.
OptimaT L-90 K Preparative Ultracentrifuge
The L-90 K instrument iscapable of generating 694,000 x g at speeds up to 90,000 rpm, the Optima L-90 K Ultracentrifuge enables you to perform more separations in less time. This versatile floor model operates with a broad range of superb rotors, including zonal and continuous flow for large-volume separations.
The L-90 K offers the reliability of a vacuum-encased induction drive, the simplicity of user-friendly, microprocessor-based control, and environmentally-friendly cooling systems that eliminate the need for CFCs and other harmful liquid refrigerants. All this makes the L-90 K the right choice for your laboratory's routine separation needs.
OptimaT TLX Personal Benchtop Ultracentrifuge
The Optima TLX features maximum performance capabilities of 657,000 x g and sample volumes ranging from 0.2 mL to 5.1 mL per tube.
System features include imbalance-tolerant, direct vacuum-encased, air-cooled drives, rotor imbalance detection, moisture-purging vacuum pump,solid-state temperature control systems; automatic rotor identification, user diagnostics; programmability with up to five step changes, ten selectable acceleration/deceleration rates and microprocessor control..
|Optima TLX (627,000 x g; 120,000 rpm)|
|Speed control||▒ 50 rpm of set speed|
|Sample imbalance tolerance||Up to 10% of volume in opposite tubes|
|Refrigeration system*||Solid state, no CFCs/ODCs*|
|Set temperature range||2░C to 40░C in 1░C increments|
|Temperature control||▒ 2░C of set|
|Ambient operating range||15░C to 35░C|
|User-settable programs||10 user programs; all user programs have up to five steps|
|Acceleration/Deceleration rates||10 Accel/11 Decel|
|Moisture-purging vacuum system||Yes|
|Heat output||0.6 kW/hr (1,750 BTU/hr)|
|Sound level 3 ft (1 m) away||< 60 dB|
|Remote control via RS-232||Optional|
|Dimensions H x W x D in (mm)||21 x 29 x 23 (533 x 737 x 584)|
|Weight||178 lb (81 kg)|
ImageQuant 350 allows high-resolution scans of monochromatic fluorescence gels, Coomassie Blue-stained gels, and other colorimetric detection methods.
ImageQuantT 350 is a high-performance 16-bit cooled CCD camera-based system designed for a wide range of monochromatic imaging applications. ImageQuant 350 is well-suited for quantitative analysis of Western blots. The system is also fully capable of imaging monochromatic fluorescent gels and membranes, CoomassieT Blue-stained gels, and other colorimetric detection methods. Cultures and microplate-based assays can also be imaged. Whereas film is valuable and convenient for certain applications, ImageQuant 350 supports a more quantitative approach for a broader range of applications without compromising sensitivity, resolution, or simplicity of operation. Most commands are performed by pushing a single button, thereby supporting many different applications in a day.
The ImageQuant 350 is a compact, easily installed system, designed for easy exchange of cameras, lenses, and filters. The standard system includes one manual fixed lens and one manual zoom lens, and the motorized system includes one motorized fixed lens and one manual zoom lens.
The standard UV transilluminator and the white light source (a fold-down table), as well as any optional light sources, are housed dust-free inside the cabinet. The UV transilluminator can be operated at either 302 nm or 365 nm at low or high intensity. For cutting sample bands from the gel, the transilluminator slides out of the cabinet.
Using ImageQuant TL analysis software with ImageQuant 350 provides high levels of automation, accuracy and reduces analysis time to a minimum.AUC
Analytical ultracentrifugation is used for the determination of molecules masses in solution. The instrument analyzes macromolecular sedimentation over a wide range of conditions such as concentration,temperature, ionic strength and pH.
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