השבחה מולקולרית של צמחים - מעבדה (0466.4001)
Plant molecular breeding- Lab


Plant molecular breeding lab, taught by Dr. Assaf Distelfeld – 3 points (1 week, 6.5 hours / day)


The course will focus on the application (theory + hands on) of molecular genetics and genomics to the development of cultivars more suited to the needs of humans. A final written exam will be conducted at the end of the course.

As lab space is limited, prerequisites include relevance, student commitment, and lecturers’ approval.

Course Schedule:

Day 1: Introduction to plant breeding
Plant breeding objectives
Germplasm resources and pools
Crop evolution (domestication + evolution under domestication)
Plant breeding methods
Laboratory – DNA extraction

Day 2: Genetics
Genetic variation
Recombination and population development
Segregating populations
Linkage drag
Polyploidy
Introduction to molecular genetics
Laboratory – PCR and gel electrophoresis – SSR markers

Day 3: Marker Assisted Selection (MAS)
MAS traits
Genetic markers
Bases and availability of markers
Laboratory – PCR and gel electrophoresis – gene specific markers

Day 4: Genetic mapping
QTL
Marker assisted selection and backcrossing
Quantitative genetics model
Laboratory – CAPS markers, SNP assay

Day 5: Breeding with Molecular Markers
Gene discovery
Reverse genetics
Mutation breeding
Biotechnology (transgenic)
Laboratory – genetic mapping
מס תלמידים: מוגבל
דרישות קדם: אין