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Three forms of gonadotropin-releasing hormone in a
perciform fish (Sparus aurata): complementary deoxyribonucleic acid
characterization and brain localization.
Gothilf Y,
Munoz-Cueto JA, Sagrillo CA, Selmanoff M, Chen TT, Kah O, Elizur A,
Zohar Y.
Center of Marine Biotechnology, University of
Maryland, Baltimore 21202, USA.
Three forms of GnRH-salmon
(sGnRH), seabream (sb-GnRH), and chicken (cGnRH-II)-have been described
in the gilthead seabream (Sparus aurata) brain, and the cDNA encoding
the sbGnRH precursor was recently isolated. In the present study, the
cDNAs encoding the sGnRH and cGnRH-II were isolated and characterized,
and the neurons producing the three GnRHs were localized in the seabream
brain. Fragments of sGnRH and cGnRH-II cDNAs were amplified by
polymerase chain reaction and used as probes to isolate the full-length
cDNAs from a brain cDNA library. The cDNA encoding the cGnRH-II
precursor is 573 nucleotides (nt) long, and the cDNA encoding the sGnRH
precursor is 1971 nt in length with an unusually long 5' untranslated
region. Specific single-strand DNA probes for in situ detection of mRNA
were designed according to nonconserved regions among the three GnRH
c-DNAs. Localization of GnRH mRNA-producing cells in the brain revealed
five distinct populations of cells: sGnRH-producing cells in the
ventromedial olfactory bulbs and the terminal nerve, sbGnRH-producing
cells in the preoptic area and the ventral thalamus, and
cGnRH-II-producing cells in the midbrain tegmentum. The discrete sites
of expression of the three forms of GnRH indicate that only sbGnRH is
directly involved in the control of gonadotropin secretion.
PMID:
8862782 [PubMed - indexed for MEDLINE]
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