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Functional Development of the Zebrafish Pineal
Gland: Light-Induced Expression of Period2 is Required for Onset of the
Circadian Clock.
Ziv L, Levkovitz S, Toyama R,
Falcon J, Gothilf Y.
Department of Zoology, The George S.
Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv,
Israel.
Abstract In zebrafish, the pineal gland is a
photoreceptive organ that contains an intrinsic circadian oscillator and
exhibits rhythmic arylalkylamine-N-acetyltransferase (zfaanat2) mRNA
expression. In the present study, we investigated the role of light and
of a clock gene, zperiod2 (zper2), in the development of this rhythm.
Analysis of zfaanat2 mRNA expression in the pineal gland of 3-day-old
zebrafish embryos after exposure to different photoperiodic regimes
indicated that light is required for proper development of the circadian
clock-controlled rhythmic expression of zfaanat2, and that a 1-h light
pulse is sufficient to initiate this rhythm. Analysis of zper2 mRNA
expression in zebrafish embryos exposed to different photoperiodic
regimes indicated that zper2 expression is transiently up-regulated by
light but is not regulated by the circadian oscillator. To establish the
association between light-induced zper2 expression and light-induced
clock-controlled zfaanat2 rhythm, zPer2 knock-down experiments were
performed. The zfaanat2 mRNA rhythm, induced by a 1-h light pulse, was
abolished in zPer2 knock-down embryos. These experiments indicated that
light-induced zper2 expression is crucial for establishment of the
clock-controlled zfaanat2 rhythm in the zebrafish pineal
gland.
PMID: 15869567 [PubMed - in process]
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