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Developmental expression of three forms of
gonadotropin-releasing hormone and ontogeny of the
hypothalamic-pituitary-gonadal axis in gilthead seabream (Sparus
aurata).
Wong TT, Gothilf Y, Zmora N, Kight KE,
Meiri I, Elizur A, Zohar Y.
Center of Marine Biotechnology,
University of Maryland Biotechnology Institute, Baltimore, Maryland
21202, USA.
To address the complexity of the origin of the GnRH
system in perciforms, we investigated the ontogenic expression of three
GnRHs in gilthead seabream. Using in situ hybridization, chicken (c)
GnRH-II mRNA-expressing cells were detected in the hindbrain at 1.5 days
postfertilization (DPF) and in the midbrain at 2 DPF and thereafter; the
hindbrain signals became undetectable after 10 DPF. Salmon (s) GnRH
mRNA-expressing cells were first seen in the olfactory placode at 3 DPF,
started caudal migration at 14 DPF, and reached the preoptic areas at 59
DPF. Seabream (sb) GnRH mRNA-expressing cells were first detected in the
terminal nerve ganglion cells (TNgc), ventral part of the ventral
telencephalon, nucleus preopticus parvocellularis, and thalamus at 39
DPF, and extended to the nucleus preopticus magnocellularis at 43 DPF,
ventrolateral hypothalamus at 51 DPF, and nucleus lateralis tuberis and
posterior tuberculum at 59 DPF. Coexpression of sbGnRH and sGnRH
transcripts was found in the TNgc. Using real-time fluorescence-based
quantitative polymerase chain reaction, transcript levels of cGnRH-II
and sGnRH were first detected at 1 and 1.5 DPF, respectively, and
increased and remained high thereafter. Transcript levels of sbGnRH
remained low after first detection at 1 DPF. Furthermore, these GnRH
expression profiles were correlated with the expression profiles of
reproduction-related genes in which at least four concomitant increases
of GnRH, GnRH receptor, gonadotropin, gonadotropin receptor, and Vasa
transcripts were found at 5, 8, 14, and 28 DPF. Our data provide an
expanded view of the ontogeny of the GnRH system and reproductive axis
in perciforms.
PMID: 15163612 [PubMed - indexed for MEDLINE]
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