LS 6500
LS6500 Liquid Scintillation Counter

LS 6500 Scintillation Counter combine advanced scintillation technology, configurability to meet your exact requirements and built-in upgradability to add features, as you need them. Value Systems are instrument configurations that meet standard requirements, H-Number Plus, our patented quench quench monitor, and Versa-RackT are standard features on both LS 6500 Value Systems. You can count samples in any combination; up to 336 standard vials (20 mL).
The isotope library C 14, H3,  I125, .S35 and P32 are permanently stored in the system.


Biolistic PDA
  Gene Transfer

 The biolistic PDS-1000/He unit. Top, main chamber containing the micro carrier launch assembly and the bombardment helium pressure gauge. The central gauge (in the left side of the instrument) monitors the vacuum within the chamber, and the 2 lower knobs adjust the vacuum flow and vent rates. The Helium metering valve is next to the main chamber.
PDS-1000 | He System
 


Transform Any Cell Type

The biolistic PDS-1000/He instrument is capable of accelerating sub cellular-sized tungsten or gold microprojectiles coated with DNA over a range of velocities and target distances necessary to optimally transform many different cell types. Particle delivery with the PDS-1000/He system is a convenient method for transforming intact cells in culture since very little pre- or post bombardment manipulation is necessary, and sample containment within the evacuated bombardment chamber offers maximum control.Biolistic bombardment allows transformation of animal cells with unique growth requirements that are not amenable to gene transfer using any other method. Reports describing how to transform cultured mammalian cells with the PDS-1000/He device (see References) show improved transient transformation of a variety of sensitive mammalian cells that are poorly transformed by chemicals, lipofection, or even electroporation. In addition, you can use less DNA and fewer cells for either transient or stable transformation with the biolistic particle delivery system. This technique is much easier and faster than the tedious task of microinjecting embryos. How the Unit WorksThe helium pressure and vacuum circuits in the PDS-1000/He system effectively accelerate the microcarriers into the target cells: After all the materials are in place, the chamber door is closed and a vacuum is applied. The vacuum reduces the frictional drag on the DNA-coated microcarriers and provides a safety interlock; the instrument cannot be activated unless a vacuum is drawn.Activating the fire switch allows helium to flow into the gas acceleration tube at a rate regulated by the helium-metering valve and monitored by the helium pressure gauge. The gas is held until the burst pressure of the rupture disk is reached. This generates a helium shock wave into the bombardment chamber.The shock wave hits the microcarrier launch assembly and propels a plastic macrocarrier holding DNA-coated microcarriers toward the target cells. A stopping screen placed between the macrocarrier assembly and the cells retains the plastic disk, while allowing the coated microprojectiles to pass through and transform the target cells.Bombardment ParametersWe recommend these settings for bombardment of a variety of cell types. Many factors affect bombardment efficiency, but most users will find it sufficient to optimize the major variables individually, then test their interactions on a limited scale.    

Bombardment Parameters

• Strain Typing-Molecular Epidemiology
• Apoptosis Assays
• DNA Damages and Repair Studies
• Large Protein Separations
• Disease Loci Mapping
• YAC, BAC, PAC, and Cosmid Mapping
• Chromosome Rearrangements
• PFLP and DNA Fingerprinting.
  
  
  
  Confocal Microscop
  LSM 510
  
  
  
  LSM 510  system is
  unmatched. It allows multi fluorescence images to be collected without compromising resolution and efficiency.
  Imagine the new system for research:
  Unprecedented flexibility for individual applications. Entirely new acquisition and processing functions that guarantee fast and efficient structural and functional analysis of your specimens. A stable and reliable system, allowing you to concentrate on the content of your research
  The ZEISS LSM system is highly integrated scanning module and fully motorized microscope

Argon Laser: 458nm , 488nm and 514nm

HeNe: 543nm and 633nm

Circular Dichroism Spectrophotometer

The Aviv Model 215 Circular Dichroism Spectrometer

Circular dichroism spectroscopy measures physical phenomena wherein molecules absorb to different extents circularly polarized light. Differences in the absorbance are directly related to the optical activity of chiral molecules.  
Circular dichroism is an essential tool of measuring secondary structure of proteins, ligand binding and macromolecular association.  Deconvolution of the spectra can give a quantitive measure of protein secondary structure.
The Aviv model 215 Circular Dichroism Spectrometer records circular dichroism as a function of wavelength, time, temperature, pH and concentration using a double monochromator containing two UV grade prisms as dispersing elements. Focusing optics allows use of micro cells. A 50 Khz photoelastic modulator produces right and left circularly polarized light.
The instrument allows secondary structure estimates, stopped-flow kinetic studies, wavelength scans, temperature scans, titration studies, total fluorescence measurements, and more. Wavelength ranges 165nm to 900nm .




Multiphor

MultiphorT II flatbed
Two-dimensional (2-D) electrophoresis is a powerful analytical technique capable of resolving thousands of individual proteins in complex biological samples. In cases where the resolution provided by larger-format systems is not required, the speed and convenience of a mini-format can be exploited.
The Multiphor T II Flatbed Electrophoresis Unit allows both the first and second dimensions of 2-D electrophoresis to be performed with the same instrument. Three second-dimension separations are run simultaneously on a single second-dimension gel, which is particularly useful for comparative studies, as all three samples experience identical separation conditions. Silver staining using the PlusOne T Silver Staining Kit, Protein; and the Hoefer T Automated Gel Stainer provides exceptionally sensitive visualization when applied to the
ExcelGel T precast gel used in this technique.
Multiphor II electrophoresis system is a versatile modular system of Pharmacia for horizontal electrophoresis, isoelectric focusing 2-D electrophoresis and electrophoretic transfer.

 

 
Axioplan
Zeiss Axioplan
Zeiss upright fluorescence microscope
The filters are:

1)UV filter, EX 365, EM 420.

 2)Green filter, EX 490 EM 525.

3)Red Filter,EX 540 EM 580.

Fluorescence microscope
DMRBE
Leica DMRBE

Leica manufactured DMRB upright microscope with magnafire 12-bit color CCD camera.
  The filters are:

1)B.G.R filter for uv, blue and green, excitation filter,  BP 400/20, Dichromatic mirror rkp   415, Suppression filter BP 465/20.

 2)DAPI excitation filter BP 340-380, Dichromatic mirror rkp 400, Suppression filter LP 425.

3) M2(Rhodamin) excitation filter BP 546/14, Dichromatic mirror  rkp 580,  Suppression filter LP 590.

 4) Gfp excitation 470/40x,  Dichromatic mirror hq495LP,  em hq 525/50m.

Gamma Counter
Packard RIASTAR

Packard RIASTAR multi well gamma counter
5 well multi well gamma counter with multi channel analyzer technology The instrument is configured with 10 detectors.
Packard RiaStar Gamma Counting Systems includes multi -channel analyzer, Instrument Performance Assessment (a self-inspecting system) and automatic protocol operating.

 

HPLC- High Performance Liquid Chromatography
Waters HPLC

The water's hplc includes 50u pumps model 626, fluorescence detector 474, PDA detector 996, 600s controller, vacuum degasses and non-cooled auto sampler 717.
The instrument is fully controlled by the millennium software.



Luminometer
TD-20/20 Luminometer

The Turner Designs model 20e is equipped with a Hamilton injector for automatic injections.
Its EPROM includes auto ranging and 2nd functions for smoothing, pre delay, delay, repeat, and continuos reading.



The most sensitive display range is 0.000 to 9.999 and the least sensitive display range is 0000 to 9999.

Microplate Reader Spectramax 190

SPECTRAmax 190

The SPECTRAmax 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The corrected absorbance is within 5% of the value obtained in a conventional spectrophotometer
The SPECTRAmax 190 microplate reader allows you to:
Use the extinction coefficient of your sample to calculate concentrations directly,
Correct for volume differences in the wells, expand the dynamic range of your assay,
Detect pipetting errors and check performance of your pipetting devices

 

Preparative Electrophoresis

  Preparative Electrophoresis

Any molecule that can be resolved in a slab gel can be purified quickly, easily, and completely using continuous elution electrophoresis. Using SDS-PAGE, native PAGE, or agarose gel electrophoresis, the Model 491 prep cell and mini prep cell isolate individual components from crude or partially purified samples. These instruments are ideal for use at any stage in a purification scheme. As a final step following chromatography or preparative isoelectric focusing, these instruments can be used to isolate a specific molecule from any contaminants remaining in the sample.
·    Conventional electrophoresis buffer systems and media are used
·    Purify nanograms to milligrams of a particular molecule
·    Using SDS-PAGE, purify molecules that differ in molecular weight by as little as 2%
·    Using native PAGE, isolate molecules that differ in isoelectric point by as little as 0.1 pH unit.
Purified molecules are automatically collected in individual liquid fractions and are immediately available for sequence analysis, crystallography, antibody production, enzyme kinetics studies, NMR, bioassays, or any other study where homogeneous bio-molecules are required. Single- and double-stranded DNA and RNA can also be purified using the prep cell.

 

Rotofor
Protein Purification Simplified

The Rotofor system fractionates complex protein samples in free solution using preparative isoelectric focusing. This nondenaturing technique rapidly isolates proteins of interest from the bulk proteins in complex biological samples. With the addition of the mini Rotofor focusing chamber, this unique system accommodates a wide range of sample volumes and sizes.

• Ideal for the isolation of uncharacterized proteins from crude or partially purified samples
• Interchangeable mini and standard focusing chambers
• Process micrograms to grams of protein in 18 to 58 milliliters of sample
• Easy set-up and short run times enable 500-fold purification in less than 3 hours
• Ceramic cooling finger dissipates heat generated during high voltage runs
• The unique membrane core stabilizes proteins in 20 focused zones and facilitates free-solution collection without mixing

 

Sequencer ABI 377 and 3100
http://www.tau.ac.il/lifesci/zabam/sequencing/

Telemeter
Ask Dr. Eli Geffen

Spectrophotometer Ultrospec 2000

Pharmacies ultrospec 2000 UV/visible spectrophotometer measures standard absorbance, concentration and transmittance and has stored parameters for DNA, RNA and oligonucleotide quantification and purity checking as well as for protein contamination measurements in nucleic acid solutions.
Kinetic is also possible with 6 cell changer.
Bandwidth of 3nm and stray light at 220nm < 0.012%T.
 
Ultracentrifuge - OptimaT LE
The Beckman optima le70 is equipped with the following rotors: SW 28, SW41, SW50.1, SW60 and 70, 70.1Ti.



The max rpm are 70,000 and max vacuum is less than 20 microns   

Elutriation Centrifuge -Avanti® J-25

The Avanti series is a centrifuge that allow the centrifugal elutriation of living cells
Cells are subjected to two forces within the separation chamber: the centrifugal field and the viscous drag of the fluid following in the opposite (centripetal) direction. Each cell tends to migrate to a zone where its sedimentation rates are balanced by the flow rate of the fluid through the separation chamber.
By increasing the flow rate of the elutriation fluid in steps or decreasing the rotor speed, successive populations of relatively homogenous cell sizes can be washed from the chamber.
 
 

Densitometer-image master

ImageMaster 1D Image Analysis Software

Image analysis is an integral part of most of today's Life Science applications and at APB we have the most comprehensive range of software products available. Under the Image Master label, we cater for all your analysis needs, from the basic documentation of a routine purity screen to the querying of entire projects of 2D gels for discovering protein expression changes.
This site covers all your analysis needs for applications which include band and spot quantitation, the study of complex band pattern matching in 1D gels, examination of lane relationships between many gels, high throughput screening techniques aimed at 2D proteomics projects and rapid comparison of macro arrays.
Combined with the large range of image capture devices we offer for all types of detection methods, we can assemble a complete system for exactly the application with which you work, whether it be DNA or proteins, arrays, 1D or 2D gels, labeled or stained.
                                             

Umax scanner and two software for densitometry developed by Pharmacia enabling quantitation of 1D gels and 2D gels

PAM 2000
"PAM-Fluorometer Family" employs the so-called Pulse-Amplitude-Modulation (PAM) measuring principle, which is unique in providing a highly selective measure of the relative chlorophyll fluorescence quantum yield. In conjunction with the so-called "saturation pulse method" of fluorescence quenching analysis PAM-2000 -Fluorometers allow rapid assessment of photosynthetic energy conversion. Measuring principle and quenching analysis
   The Portable Chlorophyll Fluorometer PAM-2000 has been developed on the basis of the well-known PAM Fluorometer. The patented pulse amplitude modulation (PAM) measuring principle is used to measure the fluorescence yield with high
Sensitivity and selectivity, whereby even strong unmodulated light (e. g. full sunlight and saturation pulses with 10000 µmol quanta ms) causes no disturbing effects.
The intensity of the measuring light is sufficiently low that it has no effect on the Photosynthesis State and the fluorescence yield. In addition to the current fluorescence yield (Ft, in continuous light) and the maximum yield (Fm', in the saturation pulse) it is also possible to determine the minimum yield (Fo', in the quasi-dark state). This information is automatically evaluated by the data acquisition system integrated in the PAM-2000 Fluorometer such that the quenching coefficients (qP and qN), the effective quantum yield (F/Fm') and the apparent electron transport rate (PAR x F/Fm') are obtained within seconds following each saturation pulse

 
IPGphor- Isoelectric Focusing System

IPGphor Isoelectric Focusing System is a dedicated instrument for performing immobilized pH gradient (IPG) IEF. IPGphor delivers rapid, high-resolution first dimension protein separations with pre-cast Immobiline Dry Strip IPG gels.
The integrated system includes a programmable 8,000 V, 1.5 mA power supply and Peltier solid-state temperature control (18-25°C ± 1 °C) for up to 12 IPG strips.
IPGphor features an innovative strip holder, made out of aluminum oxide and platinum, which allows reswelling of IPG strips and subsequent IEF separation without handling the strips between steps. For examples of results obtained using IPGphor Isoelectric Focusing System.

  

    qRTPCR

The ABI Prism® 7000 Sequence Detection System is a complete, real-time PCR system that detects and quantitates nulceic acid sequences. In real-time PCR, cycle-by-cycle detection of accumulated PCR product is made possible by combining thermal cycling, fluorescence detection, and application-specific software in a single instrument. Quantitative results are available immediately after PCR without additional purification or analysis.

Real-time, quantitative PCR applications include gene expression and pathogen detection.Post-PCR detection is also available for non-quantitative assays such as allelic discrimination (SNP detection) and plus/minus assays.

Key Features and Benefits

Scanner 428

Affymetrix 428 Array Scanner

The Affymetrix 428 Array slides  Scanner is a digital confocal laser scanning epifluorescent microscope for viewing fluorescent-dye tagged samples.  

The scanner is equipped with  two lasers : 532nm and a 635nm (cy3 and cy5).

Data Mining Tool (DMT).

 The Data Mining Tool (DMT) software provides a variety of tools for filtering and sorting GeneChip® array data enabling you to find your most significant data results quickly and easily. Key features include:

Pairwise statistical analysis . Clustering . Annotation Integration

DMT is compatible with Windows NT and Windows 2000 operating systems.

Nexus 470

Fourier transform-infrared (FT-IR)

Fourier transform infrared (FT-IR) is a technique that uses an interferometer for data collection and a digital Fourier transformation to process the data.

TheNexus FT-IR spectrometer family provides superior performance to meet the demanding infrared sampling requirements. Design excellence and smart system components ensure the Nexus will provide reproducible and accurate results. The Nexus provides real-time feedback on all aspects of sample analysis with exclusive Enhanced Synchronization Protocol (E.S.P.) technology.

PIT PIT tag (Passive Integrated Transponder) externally mounted or implanted RFID (Radio Frequency Identification) product used to positively identify animals and other objects (rocks, golf balls, driftwood, etc.) for a lifetime. Each tag is a read-only tag that is programmed to transmit a unique code only when activated. This code cannot be changed. There are tags that can be programmed but Biomark, Inc. does not offer that product nor do we recommend it for animal identification .

PIXImus

 href="http://www.gemedicalsystems.com/rad/bonedens/peripheral/piximus_spec.html">PIXImus Academic and pharmaceutical researchers commonly use the mouse for investigations of genetics, cellular physiology, and of agents affecting bone or soft-tissue composition. Bone densitometry and body composition measurement using dual-energy x-ray absorptiometry (DEXA), eliminate the need for destructive chemical analysis - a time- and labor-consuming process that requires days or weeks to complete. Bone densitometry allows the researcher the opportunity to make multiple measurements in-situ during the life of the animal. Unfortunately, DEXA densitometers using rectilinear scanning require as much as 30 minutes for a total body acquisition with relatively poor precision (>5%) and spatial resolution (>0.5mm). The long process requires careful sedation techniques and often endangers animal safety. The PIXImus densitometer provides bone mineral and body composition results from total body imaging in under 5 minutes. Fast imaging allows use of milder sedation that is safer on animals.The PIXImus allows accurate and precise measurement of bone and tissue for small animals 10-50 g (e.g.,mice, lemmings). This measured bone exhibits excellent correlation to total ashed weight (r=0.99). PIXImus uses a lower x-ray energy than that used for peripheral densitometry in humans in order to achieve contrast in the extremely low density bone. Excellent precision of BMD and %Fat makes PIXImus ideal for longitudinal studies. The standard total body focuses on the active bone area in the subcranial region. There are manual ROI's for selected regions within the total body image, such as spine and femur. /P>< /FONT>

   Microplate Luminometer

 Features

Detection limit of 3 x 10^-21 moles of luciferase using Promega's Luciferase Assay Reagent

• Linear dynamic range greater than nine decades
• Crosstalk better than 3 x 10^-5
• Dual injector
• Microplate 96-well format
• Easy to use software

  Synergy

 

When making fluorescence determinations, the Synergy HT uses a tungsten halogen lamp with interference filters:

   Default Excitation Filters No.1: POS1=340/11, POS2=485/20, POS3=530/25 and  POS4= 310/20.

   Optional Excitation Filters No.2 : POS1= 360/40, POS2= Plug , POS3=Plug    and  POS4= 540/35.

 Default   Emission Filters No1: POS1=460/40, POS2= 528/20, POS3=590/20 and POS4= 580/50.   

 Optional  Emission Filters No2: POS1=Empty, POS2=  645/15 , POS3= 400/10   and  POS4=600/40 .

 

When absorbance measurements are made, the instrument switches to a Xenon Flash Lamp and a monochromator for wavelength selection from 200 to 999 nm.
 Features

• Automatic top and bottom epifluorescence read modes optimized for both sensitive homogeneous and cell-based assays
• Software-controlled automatic probe height adjustment with built-in plate sensor to prevent probe crashes
• Scanning monochromator for wavelength scans to identify peak absorbance and provide full spectral analysis
• Luminescence measurements with top detection for best sensitivity
• Reads 6- to 384-well plate formats including PCR trays and Terasaki plates
• Well area scanning for popular cell-based assays
• Time Resolved Fluorescence capability
• USB and serial ports for easy connectivity
• Includes powerful PC software GEN 5 with exclusive PowerReports feature for custom reporting

VP-ITC

VP-ITC

Features include: 
• Fixed-in-place cells for reproducible ultrasensitive performance with low maintenance
• Active cell volume ~ 1.3 ml
• Cells fabricated from Hastelloy®, which has excellent chemical resistance properties
• Linear drive liquid delivery system incorporating a precision 250 microliter syringe
• Variable mixing speed
• Temperature range 2ºC to 80ºC
• Internal Peltier mechanism - no external heating or cooling required
• Directly measure binding constants in the range of <  10² to ~10⁹ M ^-1  
• Measures tight binding constants (greater than 10⁹ M^-1) using competitive binding techniques
• NEW!  Now includes Single Injection Method software.

 

X-ray Diffraction

 

The possibility of using powder diffraction methods to study materials was recognized shortly after the discovery of X-ray diffraction by von Laüe and Knipping in 1910. In particular, the construction of a simple powder diffractometer was described by Hull in 1917, and the instrument was used to obtain patterns from a number of simple materials such as diamond, graphite and iron. Within a few years, many others, including the Braggs and Pauling, had exploited the powder method to study a wide range of materials, including metals, minerals, and simple organic solids .

X-ray Diffraction System for Protein Crystals

 X-Ray1

1. Rigaku (manufacturer); ultraX18 (18kW rotating anode x-ray generator); Raxis-IV Image Plate area detector;

confocal "blue" OSMIC X-ray mirrors.

 

X-Ray2-NEW

2 . Rigaku (manufacturer); MicroMax^TM-007 HF (800W rotating anode microfocus x-ray generator);

R-AXIS IV⁺⁺ Image Plate area detector; VariMax^TM focusing X-ray mirrors.

LSM_META

The Scanning module of LSM 510 META

Details The unique scanning module is the core of the LSM 510 META. It contains motorized collimators, scanning mirrors, individually adjustable and positionable pinholes, and highly sensitive detectors including the META detector. All these components are arranged to ensure optimum specimen illumination and efficient collection of reflected or emitted light. A highly efficient optical grating provides an innovative way of separating the fluorescence emissions in the META detector. The grating projects the entire fluorescence spectrum onto the 32 channels of the META detector. Thus, the spectral signature is acquired for each pixel of the scanned image and subsequently can be used for the digital separation into component dyes.

The Beam Path:

1. Optical Fibers 2. Motorized collimators 3. Beam combiner 4. Main dichroic beamsplitter 5. Scanning mirrors 6. Scanning lens 7. Objective lens

8. Specimen 9. Secondary dichroic beamsplitters 10. Confocal pinhole 11. Emission filters 12. Photomultiplier 13. META detector 14. Neutral density filters

15. Monitor diode 16. Fiber out

ND1000

The NanoDrop ND-1000 UV-Vis Spectrophotometer enables highly accurate analyses of 1 ul samples with remarkable reproducibility. The patented sample retention system eliminates the need for cuvettes and capillaries which decreases the measurement cycle time. In addition, the high absorbance capability eliminates the need for most dilutions. You'll find yourself making fewer dilutions and taking more readings than ever before, essentially becoming a more quantitative laboratory.

The ND-1000 offers many benefits: Small samples: designed for 1 ul samples Large dynamic range: measures 2-3700 ng/ul< (dsDNA) on a single sample. No need to change out cuvettes or caps. High absorbance capability: 50 times that of traditional spectrophotometers No Dilutions for most samples No extra costs for consumables Full Spectrum (220-750nm) 10 second measurement time No cuvettes or capillaries Sample is recoverable Small size (size of a tissue box) Easy set up (plug and play) Software designed for life sciences Free software upgrades

FL3-11

 

FL3-11

Modular Spectrofluorimeter: single grating excitation and emission Monochromators, Datamax/32 software with GRAMS license .
SPECIFICATIONS:

 OPTICS: All reflective for focusing at all wavelengths and precise imaging for micro samples
 SOURCE: Ozone-free 45O W xenon lamp eliminates venting
 SPECTROMETERS: Plane grating Czerny-Turner design maintains focus at all wavelengths
EXCITATION: Range 200-950 nm, optimized in the UV, blazed angle 330 nm
 EMISSION: Range 200-950 nm, optimized in the visible, blazed angle 500nm
BANDPASS: Ex.& Em, 0-30 nm, continuously adjustable
WAVELENGHT ACCURACY : +/- 0.5nm
 STEP SIZE: 0.0625 -100 nm 
SCAN SPEED: 150 nm/s
 INTEGRATION TIME: From 1 ms to 160s
 EMISSION DETECTOR: Photomultiplier range 200-850 nm
 REFERENCE DETECTOR: Photodiode selected for stability
SAMPLE MODULE: T-box design to allow second detection emission channel
WATER RAMAN SIGNAL: min. 400.000cps, EX=350nm, EM=397nm, BP=5 nm, integration=1s
S/N RATIO: 4000/1 minimum Thermocouple Drive Single Position-Includes controller, software, stirring mechanism and nitrogen cord for flushing: Range: -10 to Required serial port in the PC. Polarization Accessory L-format, fully automated dual polarizer. Located at both the entrance and exit of the T-box for optimum extinction ratio and Sensitivity. Sample, Compartment, Accessory, Electronics Necessary to control and manipulate automated sample chamber accessories.

FLA 5100

 

FLA 5100

Radioisotopic Imaging

The system ensures radioisotopic imaging with high sensitivity, which comes from Fujifilm's unique and reliable technologies. Imaging plate (IP) consists of a radiosensitive layer of photostimulable phosphor crystals on a polyester support. The IP accumulates irradiated radiation energies on exposure for further scanning excitation process. The innovation and ergonomic design provides a broad range of advantages.

Fluorescence

FLA-5100 has three kinds of excitation laser beams: 473nm, 532nm and 635nm.  The system has four  filters (510nm, 575nm, 665nm,  and a filter for IP) for upgrading flexibility.

MALS 

DAWN^® HELEOST II

 

18-angle light scattering detector for the measurement of absolute molecular weight, size, and conformation of macromolecules in solution.  The DAWN^® HELEOST II may be used in batch mode (off-line) or connected on-line to an HPLC/HPSEC/AFFF, etc.. The DAWN^® HELEOST II utilizes a 120mW solid-state laser operating at 658 nm and also has on-board digital signal processing hardware for up to four external devices such as RI and/or UV detectors. Also contains an analog output from the 90° detector for interfacing to strip chart recorders. The DAWN^® HELEOST II has a gorgeous 64,000 color LCD display, thermostatic control options, depolarization options, and the ability to interface to high temperature GPC systems like the PL210 and Waters Alliance 2000. On-board analog-to-digital conversion with Ethernet communication for data acquisition

Optilab T-rEX

 

Optilab Refractive Index Detector

The Optilab® rEX (refractometer with EXtended range) is an Refractive Index detector with 256 times the detection power and up to 50 times the dynamic range of every RI detector in existence today. Using a combination of cutting-edge semiconductor photodiode technology and proprietary computer algorithms, the rEX presents a host of instrument firsts.
For example, there are no range or gain settings. The full range of instrument detection is always present, and the full sensitivity exists over the entire range. 
The rEX also has a flow cell with a total volume of only 7.4 uL -- 25% less than the leading refractometers available today. This translates into minimized band broadening and better temperature stability.
The Optilab® rEX can also do something no other on-line Refractive Index detector can: it can measure the absolute refractive index of a solution. In addition, it can measure the dn/dc of a solvent at the same wavelength of light as the light scattering instrument. And just to make sure it can't be matched, you can replace the light source in the rEX for other wavelengths (e.g. 690, 633, 488, etc.)!
The new Optilab® rEX shares the ability its predecessor (the Optilab® DSP) to go to below ambient temperatures as easily as above ambient. No hassle temperature control can be programmed down to 4°C or as high as 50°C. Compatible with all HPLC systems.

FACS 

BD Biosciences

 

Two Lasers: 488 and 630

Software: Flow Jow

ChirascanT Circular Dichroism Spectrometer

Applied Photophysics

Specifications

Light source	150W Xe arc, air-cooled 150W Hg-Xe arc, air-cooled (optional)
Monochromator	F/7 double prism, both dispersive, both polarising
Wavelength accuracy	±0.2 nm (170nm - 400nm), ±0.5 nm (400nm - 900nm)
Wavelength precision	±0.05 nm (170 - 400 nm), ±0.1 nm (400 - 800 nm)
Wavelength resolution	0.1nm throughout range.
Bandwidth	0 to 2nm at 160nm, 0 to 4nm at 180nm, 0 to >10nm at >250nm
Wavelength range	165nm - 1360nm. Detector option for >900nm
Stray light	<5ppm at 200nm, <8ppm at 180nm
Baseline stability	±0.02 m o /hr
Scanning modes	Discrete sampling step-scan, Adaptive sampling step-scan
Scanning speeds	Dependent upon step-scan resolution approximately 500nm min-1 at 0.5nm step
Kinetic modes	Linear timebase, Split-linear timebases, Logarithmic timebase
Response time	Not applicable. Chirascan uses the superior method of digital oversampling which eliminates the risk of damage to data.
CD full scale	±1500 m
CD resolution	64 bit
RMS noise	0.015m ° at 185nm, 0.012m ° at 200nm, 0.015m ° at 500nm, (1nm bandwidth, 16s oversampling (integration)) 0.007m ° at 185nm, 0.007m ° at 200nm, 0.008m ° at 500nm, (2nm bandwidth, 16s oversampling (integration))
Data channels	Internal: 2 standard; up to 16 optional. External (standard): 6 inputs for temperature (multiplexed); 3 analogue inputs; plenty of digital I/O.

OptimaT L-90 K Preparative Ultracentrifuge  

The L-90 K instrument iscapable of generating 694,000 x g at speeds up to 90,000 rpm, the Optima L-90 K Ultracentrifuge enables you to perform more separations in less time. This versatile floor model operates with a broad range of superb rotors, including zonal and continuous flow for large-volume separations. 

The L-90 K offers the reliability of a vacuum-encased induction drive, the simplicity of user-friendly, microprocessor-based control, and environmentally-friendly cooling systems that eliminate the need for CFCs and other harmful liquid refrigerants. All this makes the L-90 K the right choice for your laboratory's routine separation needs.

OptimaT TLX Personal Benchtop Ultracentrifuge

The Optima TLX features maximum performance capabilities of 657,000 x g and sample volumes ranging from 0.2 mL to 5.1 mL per tube.

System features include imbalance-tolerant, direct vacuum-encased, air-cooled drives, rotor imbalance detection, moisture-purging vacuum pump,solid-state temperature control systems; automatic rotor identification, user diagnostics; programmability with up to five step changes, ten selectable acceleration/deceleration rates and microprocessor control..

Specifications

	Optima TLX (627,000 x g; 120,000 rpm)
Speed control	± 50 rpm of set speed
Sample imbalance tolerance	Up to 10% of volume in opposite tubes
Drive cooling*	Air-cooled*
Refrigeration system*	Solid state, no CFCs/ODCs*
Set temperature range	2°C to 40°C in 1°C increments
Temperature control	± 2°C of set
Ambient operating range	15°C to 35°C
User-settable programs	10 user programs; all user programs have up to five steps
Acceleration/Deceleration rates	10 Accel/11 Decel
Moisture-purging vacuum system	Yes
Heat output	0.6 kW/hr (1,750 BTU/hr)
Sound level 3 ft (1 m) away	< 60 dB
Remote control via RS-232	Optional
HEPA filter	Optional
Dimensions H x W x D in (mm)	21 x 29 x 23 (533 x 737 x 584)
Weight	178 lb (81 kg)

Rotors:

• TLA-120.2 Rotor, Fixed Angle, Titanium, 10 x 2.0 mL, 120,000 rpm, 627,000 x g.
• TLA-120.1 Rotor Package, Fixed Angle, Titanium, 14 x 0.5 mL, 120,000 rpm, 627,000 x g.

• TLA-100 Rotor, Fixed Angle, Titanium, 20 x 0.2 mL, 100,000 rpm, 436,000 x g.

• TLS-55 Rotor, Swinging Bucket, 4 x 2.2 mL, 55,000 rpm, 259,000 x g.

ImageQuant 350
 
ImageQuant 350 allows high-resolution scans of monochromatic fluorescence gels, Coomassie Blue-stained gels, and other colorimetric detection methods.

ImageQuantT 350 is a high-performance 16-bit cooled CCD camera-based system designed for a wide range of monochromatic imaging applications. ImageQuant 350 is well-suited for quantitative analysis of Western blots. The system is also fully capable of imaging monochromatic fluorescent gels and membranes, CoomassieT Blue-stained gels, and other colorimetric detection methods. Cultures and microplate-based assays can also be imaged. Whereas film is valuable and convenient for certain applications, ImageQuant 350 supports a more quantitative approach for a broader range of applications without compromising sensitivity, resolution, or simplicity of operation. Most commands are performed by pushing a single button, thereby supporting many different applications in a day.

The ImageQuant 350 is a compact, easily installed system, designed for easy exchange of cameras, lenses, and filters. The standard system includes one manual fixed lens and one manual zoom lens, and the motorized system includes one motorized fixed lens and one manual zoom lens.

The standard UV transilluminator and the white light source (a fold-down table), as well as any optional light sources, are housed dust-free inside the cabinet. The UV transilluminator can be operated at either 302 nm or 365 nm at low or high intensity. For cutting sample bands from the gel, the transilluminator slides out of the cabinet.

Using ImageQuant TL analysis software with ImageQuant 350 provides high levels of automation, accuracy and reduces analysis time to a minimum.

AUC

Analytical ultracentrifuge

Analytical ultracentrifugation is used for the determination of molecules masses in solution. The instrument analyzes macromolecular sedimentation over a wide range of conditions such as concentration,temperature, ionic strength and pH.   Sedimentation velocity and sedimentation equilibrium experiments can be done with the instrument.    The operating methodology measures the relative change in the distribution of molecular weights, providing an efficient way to measure heterogeneity, stoichiometry and self-associating systems. The XL-A/XL-I's measurements are based on the first principles of thermodynamics and hydrodynamics, no standards or calibrations are required.

All data and images were taken from the companies sites.

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