Ph.D.:	Tel Aviv University, Israel, 1978
Phone:	(Office) +972-3-640-9377 (Lab) +972-3-640-9845
E-mail:	adina@post.tau.ac.il
Room#:

Research Interests

My research focuses on the function and biological role of the molecular chaperones belonging to the immunophilins in plants. More specifically, I study the function of the FK506 binding proteins in wheat and Arabidopsis. We have found that these proteins are essential for the proper functioning protein folding and fertility of the plants.

The FKBPs are conserved from bacteria to man and plants and are involved in cell differentiation and proliferation. These family of proteins have been found to be involved in the immune response in humans, to affect cell division in yeast, affect cell growth and differentiation in plants and protein folding in most organisms.

In plants we study their mode of action by using genetic and molecular tools by producing mutants and transgenic plants. The acronym of this protein family is FKBP which stands for FK506 Binding Proteins . FK506 is an immunosuppresive drug used in medicine and these proteins bind the drug. All the members of this family possess peptidyl prolyl cis trans isomerase activity , affecting the folding of other proteins and some proteins behave as molecular chaperones.

We study the mode of action of FKBPs in wheat ,rice and arabidopsis. Plants which loose the FKBP become heat stress sensitive. We have found that 2 genes of Arabidopsis ROF1 and ROF2 are involved in heat stress response by their interaction with HSP90 and the transcription factor HSFA2. They form complexes that regulate expression of the small heat stress proteins . These proteins are responsible for the heat stress response.At present we follow in depth the mode of function of the complex ROF1, HSP90, HSFA2 in vitro and in vivo

Selected Publications

Kurek, I., E. Herman, N. Erel, K. Aviezer, O. Blecher & A. Breiman , 1999. The wheat peptidyl prolyl isomerase FKBP77 is heat induced and developmentally regulated Plant Physiol . 119, 693-704.

Kurek, I. , A. Harvey, D.M. Lonsdale & A. Breiman 2000. Molecular analysis of the wheat FKBP73 promoter . Plant Mol. Biol . 42, 489-497.

Kurek, I. , R. Dulberger, D. Sudhakar , P. Christou & A. Breiman , 2001.Deletion of the C- terminal 138 amino acids abrogates calmodulin binding, dimerisation and male fertility in transgenic rice . Plant Mol. Biol . 48, 369-381.

Harell J, Kurek I , Breiman A , Radanyi C, Renoir JM, Pratt WB & Galigniana MD 2002 .The Hsp90-binding immunophilins of wheat,wFKBP73 and wFKBP77 interact with cytoplasmic dynein via their peptidyl prolyl isomerase domains Biochemistry 17,5581-5587

. Breiman A & I. Camus 2002.The involvement of the mammalian and plant FK506 binding proteins in development (FKBPs) Transgenic Research 11,321-335.

Kurek I. , F. Pirkl, E.Fischer, J. Buchner & A. Breiman , 2002. The wheat FKBP73 functions in vitro as a molecular chaperone independent of its peptidyl prolyl cis -trans isomerase activity. Planta 215,119-126

Dwivedi R, Breiman A , Herman E, 2003. Differential distribution of the cognate and heat-stress-induced isoforms of high M r cis-trans prolyl peptidyl isomerase (FKBP) in the cytoplasm and nucleoplasm J . Experimental Botany 393, 2679-2689.

Magiri E, Pisanti O., Breiman A 2006 The rice large FKBPs are differentially expressed during development and heat stress Plant Sciences , 170 ,695-704

Aviezer-Hagai K, Skovorodnikova J, Galigniana M, Farchi-Pisanti O, Maaayan E, Bocovza S, Efrat Y, von Koskull –Doring P, Ohad N, Breiman A 2007 Arabidopsis immunophilins ROF1( At FKBP62) and ROF2( At FKBP65) exhibit tissue specificity are heat stress induced and bind HSP90. Plant Mol. Biol 63,237-255

.Meiri D & Breiman A 2009 Arabidopsis ROF1(FKBP62) modulates thermotolerance by interacting with HSP90.1 and affecting accumulation of HSfA2 –regulated sHSPs Plant J .59, 387-399

Meiri D,Tazat K, Farchi –Pisanty O, Aviezer –Hagai K, Avni A, Breiman A 2010 Involvement of Arabidopsis ROF2(FKBP65) in thermotolerance. Plant

Mol. Biol 72:191-203

Full Publications

Books

Galun, E. & A. Breiman, 1997. Transgenic plants. Ed. Chiang, Y.K., Imperial College Press, Singapore, London.

Publications from last five years

Kurek, I., E. Herman, N. Erel, K. Aviezer, O. Blecher & A. Breiman , 1999. The wheat peptidyl prolyl isomerase FKBP77 is heat induced and developmentally regulated. Plant Physiol. 119, 693-704.

Kurek, I., A. Harvey, D.M. Lonsdale & A. Breiman , 2000. Molecular analysis of the wheat FKBP73 promoter . Plant Mol. Biol . 42,489-497.

Carol R , Breiman A , Erel N, Vittorioso P & Bellini C. 2001. Pasticcino 1 (At –FKBP70) is a nuclear –localised immunophilin required during Arabidopsis thaliana embryogenesis Plant Sciences 161, 527-535.

Kurek , I., R. Dulberger, D. Sudhakar, P. Christou & A. Breiman , 2002. Deletion of the C terminal 138 amino acids abrogates calmodulin binding, dimerisation and male fertility in transgenic rice . Plant Mol.Biol .48, 369-381.

Kurek I., F. Pirkl, E. Fischer, J. Buchner & A. Breiman , 2002. The wheat FKBP73 functions in vitro as a molecular chaperone independent of its peptidyl prolyl cis -trans isomerase activity. Planta 215,119-126.

Kurek. I., E. Stoger, R. Dulberger, P. Christou & A. Breiman 2002. Over expression of the wheat FKBP77 and the heat induced FKBP77 in the transgenic wheat reveals different function of the two isoforms Transgenic Research 11,373-379.

Harell J, Kurek I , Breiman A , Radanyi C, Renoir JM, Pratt WB, Galigniana MD 2002 The Hsp90-binding immunophilins of wheat,wFKBP73 and wFKBP77 interact with cytoplasmic dynein via their peptidyl prolyl isomerase domains Biochemistry 17,5581-5587.

CV

Academic Background
Period	Institute	Subject	Degree	Awarded
1964-1969	Tel Aviv University, Israel	Microbiology	M.Sc.	1969
1972-1977	Tel Aviv University, Israel	Plant Physiology	Ph.D.	1978

Academic and Professional Experience
Period	Institute	Department	Rank/Function
1979-1981	Weizmann Institute of Science, Rehovot, Israel	Plant Genetics	Post doctoral fellow
1981-1983	National Cancer Institute	Biochemistry	Post doctoral fellow
	NIH, Bethesda, MD, USA	Fogarty fellowship
1983-1987	Tel Aviv University, Israel	Botany	Lecturer
1987-1993	Tel Aviv University, Israel	Botany	Senior Lecturer
1988	Plant Breeding Institute	Molecular Genetics	Short sabbatical
1990-1991	U.S.Department of Agriculture, Bethesda, MD	Plant Molecular Biology	Sabbatical fellow
1993- 2002	Tel Aviv University, Israel	Botany	Associate Professor
1997-2000	Tel Aviv University, Israel	Plant Sciences	Head of Department
since-2002	Tel Aviv University, Israel	Plant Sciences	Professor
since-2005	Tel Aviv University, Israel		Advisor to the President of TAU on gender issues
since-2005	Tel Aviv University, Israel		Head of the special program for outstanding students
since-2006	Tel Aviv University, Israel		Head of the Institute for cereal crops Improvment

Academic and Professional Awards - Fellowships
Year	Institute
1981-1983	National Institute of Health Bethesda, MD, USA	Fogarty fellowship	post-doctoral
1984	Plant Breeding Institute, Cambridge, UK	Short term	EMBO fellowship
1986	Plant Breeding Institute, Cambridge, UK	Short term	EMBO fellowship