Personal Information

Education

Academic Positions

Administrative and Financial Responsibilities

Research Interests

1.       Biomedical applications of enzymes and cells.

2.       Protein Nano-biotechnology: protein mediated biotemplating for the integration of biologically active protein-metal hybrid with microelectronics and development of new composite materials.

3.       Bioprocessing aspects of biocatalysis

Group members

Sefi Vernick, PhD Student

Carmit Ophir, PhD Student

Gil Mor, PhD Student

Michal Buch, PhD Student

Tal Yoetz, PhD Student

Hadar Ben-Yoav, PhD Student

Hefzi Ragunes, PhD Student

Dolev Katz, Msc Student

Tal Amzel, Msc Student

Yael Dror, Senior research assistant & Labratory mamanger

Selected Publications in the past 10 years

Publications in refereed journals

A. Freeman, S. Abramov and G. Georgiou "Site-protected fixation and immobilization of E. coli cells displaying surface anchored b-lactamase". Biotechnology and Bioengineering. 62, (1999), 155-160.

T. de Lumley-Woodyear, C.N. Campbell, E. Freeman, A. Freeman, G. Georgiou and A. Heller "Rapid amperometric verification of PCR amplification of DNA", Analytical Chemistry,71, (1999), 535-538.

N. Dotan, D. Arad, F. Frolow and A. Freeman "Self -­assembly of a tetrahedral lectin into predesigned diamond-like protein crystals". Angewandte Chemie Int. Ed. 38, (1999), 2363-2366.

N. Dotan, N. Cohen, O. Kalid and A. Freeman "Supramolecular assemblies made of biological macromolecules" In: "Nanosurface chemistry", M. Rosoff, Ed. Marcel Dekker. (2001) pp 461-471.

A. Dukler and A. Freeman "In situ removal of ketoses by immobilized 3-amino phenyl boronic acid: effect of immobilization method on pH profile". Biotechnology and Bioengineering, 75, (2001), 25-28.

N. Cohen, S. Abramov, Y. Dror and A. Freeman "In vitro enzyme evolution: the challenge of isolating the one in a million". Trends in Biotechnology 19 (2001) 507-510.

Y. Schacham-Diamand, A. Inberg, Y. Sverdlov, N. Croitoru, H. Moskovich and  A. Freeman "Electroless processes for micro and nano-electronics". Electrochimica acta 48, (2003) 2978-2996.

A. Freeman, N. Cohen-Hadar, S. Abramov, R. Modai-Hod, Y. Dror and G. Georgiou "Screening of large protein libraries by 'cell immobilized on adsorbed bead' approach". Biotechnology and Bioengineering, 86, (2004), 196-200.

T. Yaakobi, D. Rot, Y. Chen and A. Freeman "Streaming of proteolytic enzymes solutions for wound debridement: a feasibility   study". Wounds 16, (2004) 193-197

C. Omero, Y. Dror and A. Freeman "Trichoderma spp antagonism to the dermatophyte Trichophyton rubrum:  implications in treatment of onychomycosis". Mycopathologia, 158, (2004), 173-180.

N. Cohen-Hadar, Y. Wine, E. Nachliel, D. Huppert, M. Gutman, F. Frolow and A. Freeman  "Monitoring the stability of crosslinked protein crystals biotemplates: a feasibility study". Biotechnology and Bioengineering. 94 (2006), 1005-1011.

J. S. Dordick and A. Freeman "Biocatalysis as a discovery tool: from nanoscale to high-throughput and beyond". Current opinion in Biotechnology, 17 (2006) 559-561.

S. Lagziel-Simis, N. Cohen-Hadar, H. Moscovich-Dagan, Y Wine and A. Freeman "Protein-mediated nanoscale biotemplating". Current  opinion in Biotechnology, 17 (2006) 569-573.

H. Dagan-Moscovich, N. Cohen-Hadar, C. Porat, J. Rishpon, Y. Shacham-Diamand, A. Freeman "Nanowiring of the catalytic site of novel molecular enzyme-metal hybrids to electrodes". Journal of Physical Chemistry C 111(2007) 5766-5769.

T. Yaakobi, N. Cohen-Hadar, H. Yaron, E. Hirszowicz, Y. Simantov, A. Bass, A. Freeman "Wound debridement by continuous streaming of proteolytic enzyme solutions: Effects on experimental wound model in porcine".  Wounds, 19 (2007) 192-200.

Y. Wine, N. Cohen-Hadar, A. Freeman, F. Frolow "Elucidation of the mechanism and end products of glutaraldehyde crosslinking reaction by X-ray structure analysis". Biotechnology and Bioengineering (2007) 98, 711-718.

H. Ben Yoav, A. Freeman "Enzymatically attenuated in situ release of silver ions to combat bacterial biofilms: a feasibility study". Journal of Drug Delivery Science and Technology (2008) 18, 25-29.

 H. Ben Yoav, Y. Shacham-Diamand, A. Freeman, A. Biran, R. Pedhazur, S. Belkin, S. Buchinger, G. Reifferscheid " A novel microfluidic whole cell biosensor based on electrochemical detection for water toxicity analysis". ECS Transactions (2008) 16, 187-197.

N. Cohen-Hadar, Y. Wine, S. Lagziel-Simis, Y. Dror, F. Frolow, A. Freeman "Protein crystal mediated biotemplating". Journal of Porous Media (2009) 12, 213-220.

 S. Vernick, H. Moscovich-Dagan, C. Porat-Ophir C, J. Rishpon, A. Freeman, Shacham- Diamand Y "Directed metallization of single enzyme molecules with preserved enzymatic Activity".  IEEE transactions in Nanotechnology (2009) 8, 95-99.

 Y. Wine, N. Cohen-Hadar, R. Lamed, A. Freeman, F. Frolow "Modification of protein crystal packing by systematic mutations of surface residues:  implications on biotemplating and crystal porosity". Biotechnology and Bioengineering, (2009) 104, 444-457.

 S. Vernick, A. Freeman, J. rishpon, Y. Shacham-diamand " Direct biopsy screening of colorectal cancer by electrochemical biosensor". ECS Transactions (2009) 19, 61-68.

 H. Ben Yoav, N. Cohen-Hadar, A. Freeman "Biomedical implications of the porosity of microbial biofilms" In: Porous Media: Applications in biological systems and biotechnology". K. Vafai, Ed. Taylor and Francis, Boca Raton, FA, USA. (2009), In press.

Textbooks

A. Freeman and S. Abramov "Chapters in Biochemistry", Israeli Ministry of Education and Center for Technological Education - Holon; 209 pp, 1994. In Hebrew.

A. Freeman and S. Abramov "Biotechnological Processes", Israeli Ministry of Education and Pedagogic & Technological Center - "Amal"; 220pp, 1996. In Hebrew.

A. Freeman "Biotechnology Principles": An advanced course in 6 units of the Open University of Israel (1998, in Hebrew):

Selected Patents for past ten years

A. Freeman "Device for controlled enzymatic removal and retrieval of tissue". US Patent Application no 09/915,518 (27.7.01)

A. Freeman, R. Segal and Y. Dror "Method and compositions for treating fungal infections". US Patent Application no 60/306,857 (23.7.01); PCT US 02 23252.

M. Liperman, E. Herschovitz, A. Freeman " Apparatus and methods for enzymatic debridement of skin lesions". US Patent app. No 11/493,381 (27.1.05).

N. Hadar , A. Freeman. "Stable enzymatic preparations and methods of using thereof".  PCT/IL2006/001492 (27.12.06).

A. Freeman, Y. Shacham-Diamand, H. Dagan, N. Hadar " Metallization of proteins and cells with preserved biological activity". US patent application no 60/646,648 (26.1.05)

A. Freeman, Y. Shacham-Diamand, S. Vernick, H. Dagan "Directed metallization of single enzyme molecules with preserved enzymatiy activityII". US patent application no 60/682,006 (18.5.05)

N. Cohen-Hadar, A.Freeman" Antiseptic compositions and uses thereof". US Patent Application 61/151,539 (11.2.2009).