| Ph.D.: |
Tel
Aviv University 1970 |
| Phone: |
Tel:
972 - 3 - 6406017
Fax: 972 -3 - 6406018
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| E-mail: |
nelson@post.tau.ac.il
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| Room#: |
Sherman
Building, Room 530 |
| Katzir
Workshop |
http://www.tau.ac.il/lifesci/katzir-workshop.html
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Research Interests
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Molecular
biology of membrane proteins
Our lab concentrates
on the study of membrane-proteinsWe study the molecular
mechanisms involved in transport and energy transduction.
The five main subjects that we are currently engaged
in are:
- Structure, function and molecular biology of photosystem
I and other membrane complexes in higher plant chloroplasts.
We solve the structure of plant PSI super-complex
including its four light-harvesting complexes at
3.4 Å resolution.
- Biochemistry and molecular biology of neurotransmitter
transporters. Some of the neurotransmitter transporters
that we study have been implicated in epilepsy,
drug addiction, depression and several other neurological
diseases. We aim at the elucidation of their structure.
- Function of metal-ion transporters which are involved
in resistance or sensitivity of mammalian cells
to microorganisms. We have proposed that a family
of metal-ion transporters, discovered in our laboratory,
can be targeted in the aim of developing new concepts
for counteracting bacterial infection, as well as
for treatments of hereditary disorders.
- The biology of Vacuolar H+-ATPase (V-ATPase) in
yeast, plants and mammalian cells which is vital
for every eukaryotic cell and functions in numerous
processes including pH regulation in the kidney
and bone resorption by osteoclasts. We solved the
structure of V-ATPase subunit C at 1.75 Å resolution
and aim at solving the structure of several other
subunits of this important complex.
- We invented heterologous expression system of
eukaryotic membrane proteins in E. coli and aim
at solving the structure of the expressed proteins.
The common
denominator to all these subjects is the use of recombinant
DNA technology, biochemistry, structural biology and
genetics to unravel the structure and function of
membrane proteins.
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Selected Publications
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(1990-)
- Nelson, H. and Nelson, N. (1990) Disruption of
genes encoding subunits of yeast vacuolar H+-ATPase
causes conditional lethality. Proc. Natl. Acad.
Sci. USA 87: 3503-3507. (pdf)
- Nelson, H., Mandiyan, S. and Nelson , N. (1990)
Cloning of the human brain GABA transporter. FEBS
Lett. 269: 181-184.
- Nelson, N. (1991) Structure and pharmacology
of the proton-ATPases. TIPS 12: 71-75.
- Nelson, N. (1992) Evolution of organellar proton-ATPases.
Biochim. Biophys. Acta 1100: 109-124.
- Bü ttner, M., Xie, D.-L., Nelson, H., Pinther,
W., Hauska, G. and Nelson, N. (1992) Photosynthetic
reaction center genes in green sulfur bacteria and
in Photosystem I are related. Proc. Natl. Acad.
Sci. USA 89: 8135-8139. (pdf)
- Liu, Q.-R., Mandiyan, S., Nelson, H. and Nelson,
N. (1992) A family of genes encoding neurotransmitter
transporters. Proc. Natl. Acad. Sci. USA (pdf)
89: 6639-6643.
- Liu, Q.-R., López-Corcuera, B., Nelson, H., Mandiyan,
S. and Nelson, N. (1992) Cloning and expression
of a cDNA encoding the transporter of taurine and
-alanine in mouse brain. Proc. Natl. Acad. Sci.
USA 89: 12145-12149. (pdf)
- Liu, Q.-R., Ló pez-Corcuera, B., Mandiyan, S.,
Nelson, H. and Nelson, N. (1993) Molecular characterization
of four pharmacologically distinct GABA transporters
in mouse brain. J. Biol. Chem. 268: 2106-2112. (pdf)
- Liu, Q.-R., Ló pez-Corcuera, B., Mandiyan, S.,
Nelson, H. and Nelson, N. (1993) Cloning and expression
of a spinal cord and brain-specific glycine transporter
with novel structural features. J. Biol. Chem. 268:
22802-22808. (pdf)
- Supek, F., Supekova, L. and Nelson, N. (1994)
Features of vacuolar H+-ATPase revealed by yeast
suppressor mutants. J. Biol. Chem. 269: 26479-26485.
(pdf)
- Jursky, F. and Nelson, N. (1995) Localization
of glycine neurotransmitter transporter (GLYT2)
reveals correlation with the distribution of glycine
receptor. J. Neuro. Chem. 64: 1026-1033. (pdf)
- Nelson, H., Mandiyan, S. and Nelson, N. (1995)
A bovine cDNA and a yeast gene-VMA8 encoding subunit
D of the vacuolar H+-ATPase. Proc. Natl. Acad. Sci.
USA 92: Proc. Natl. Acad. Sci. USA 92: 497-501.
(pdf)
- Tamura, S., Nelson, H., Tamura, A. and Nelson,
N. (1995) Short external loops as potential substrate
binding sites of GABA transporters. J. Biol. Chem.
270: 28712-28715. (pdf)
- Supek, F., Supekova, L., Nelson, H. and Nelson,
N. (1996) A yeast manganese transporter related
to the macrophage protein involved in conferring
resistance to mycobacteria. Proc. Natl. Acad. Sci.
USA 93: 5105-5110. (pdf)
- Supek, F., Supekova, L., Nelson, H. and Nelson,
N. (1997) Function of metal-ion homeostasis in cell
divison cycle, mitochondrial protein processing,
sensitivity to mycobacterial infection and brain
functions. J. Exp. Biol. 200: 321-330. (pdf)
- Orgad S., Nelson, H., Segal, D. and Nelson, N.
(1998) Metal ions suppress the abnormal taste behavior
of the Drosophila mutant malvolio. J. Exp. Biol.
201: 115-120. (pdf)
- Nelson, N. (1998) The family of Na+/Cl- neurotransmitter
transporters. J. Neurochem. 71: 1785-1803. (pdf)
- Nelson, N. and Harvey, W.R. (1999) Vacuolar and
plasma membrane V-ATPases. Phys. Rev. 79: 361-385.
(pdf)
- Nelson, N. (1999). Metal-ion transporters and
homeostasis. EMBO J. 18: 4361-4371. (pdf)
- Cohen, A., Perzov, N., Nelson, H. and Nelson,
N. (1999). A novel family of yeast chaperons involved
in the distribution of V-ATPase and other membrane
proteins. J. Biol Chem. 274: 26885-26893. (pdf)
- Grossman, T.R., Luque, J.M. and Nelson, N. (2000).
Identificatioin of a ubiquitous family of membrane
proteins and their expression in mouse brain. J.
Exp. Biol. 203: 447-457. (pdf)
- Cohen, A., Nelson, H. and Nelson, N. (2000).
The family of SMF metal-ion transporters in yeast
cells. J. Biol. Chem. 275: 33388-33394. (pdf)
- Perzov, N., Nelson, H. and Nelson, N. (2000).
Altered distribution of the yeast plasma membrane
H+-ATPase as a feature of vacuolar H+-ATPase null
mutants. J. Biol. Chem. 275: 40088-40095. (pdf)
- Nelson, N. and Ben-Shem, A. (2002). Photosystem
I reaction center: Past and future . Photosyth.
Res. 73: 193-206 (pdf)
- Nelson, N., Sacher, A. and Nelson, H. (2002)
The significance of molecular slips in transport
systems. Nat. Rev. Mol. Cell Biol. 3: 876-881 (pdf)
- Cohen, A., Nevo, Y. and Nelson, N. (2003). The
first external loop of the metal-ion transporter
DCT1 is involved in metal-ion binding and specificity.
Proc. Natl. Acad. Sci. USA 100, 10694-10699 (pdf)
- Ben-Shem, A. Frolow, F. and Nelson, N. (2003).
The crystal structure of plant photosystem I. Nature
426, 630-635 (pdf)
- Ben-Shem, A. Frolow, F. and Nelson, N. (2004).
Evolution of Photosystem I – from Symmetry through
Pseudosymmetry to Assymmetry. FEBS Lett. 564, 274-280
(pdf)
- Nelson, N. and Ben-Shem, A. (2004). The complex
architecture of oxygenic photosynthesis. Nat. Rev.
Mol. Cell Biol. 5, 971-982 (pdf)
- Drory, O., Frolow, F. and Nelson, N. (2004). Crystal
structure of yeast V-ATPase subunit C reveals its
stator function. EMBO Rep. 5, 1148-1152 (pdf)
- Nevo, Y. and Nelson, N. (2004). The Mutation F227I
Increases the Coupling of Metal Ion Transport in
DCT1. J. Biol. Chem. 279, 53056-53061 (pdf)
- Cohen-Kfir, E., Lee, W., Eskandari, S. and Nelson,
N. (2005). Zinc Inhibition of the -Aminobutyric
Acid Transporter-4 (GAT4) Reveals a Novel Link between
Excitatory and Inhibitory Neurotransmission. Proc.
Natl. Acad. Sci. USA 102, 6154-6159 (pdf)
- Junge, W. and Nelson, N. (2005). Nature's rotary
electromotors. Science 308, 642-644 (pdf)
- Nelson, N. and Ben-Shem, A. (2005). The structure
of photosystem I and evolution of photosynthesis.
BioEssays 27, 914-922. (pdf)
- Nelson, N. and Yocum, C. (2006). Structure and
Function of Photosystems I and II. Annu. Rev. Plant
Biol. 57, 521-565. (pdf)
- Amunts, A., Drory, O. and Nelson, N. (2007). The
structure of a plant photosystem I supercomplex
at 3.4 Å resolution. Nature 447, 58-63. (pdf)
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